{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T13:47:27Z","timestamp":1776088047086,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,11,15]],"date-time":"2019-11-15T00:00:00Z","timestamp":1573776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2019,11,15]],"date-time":"2019-11-15T00:00:00Z","timestamp":1573776000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Despite having chronic gastritis, most people infected by\n                      <jats:italic>Helicobacter pylori<\/jats:italic>\n                      (\n                      <jats:italic>H.\u00a0pylori<\/jats:italic>\n                      ) are asymptomatic and have no specific clinical signs and symptoms.\n                      <jats:italic>H.\u00a0pylori<\/jats:italic>\n                      infection can be diagnosed by several detection methods. Giemsa stain and rapid urease test (CLO test) are the most performed tests of\n                      <jats:italic>H.\u00a0pylori<\/jats:italic>\n                      infection at first\u2010line clinical examination because of their simplicity and reliability. However, the sensitivity of CLO test is significantly reduced in patients with atrophic gastritis and intestinal metaplasia, and the weaknesses of Giemsa stain are higher cost and time\u2010consuming.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The Giemsa stain was modified in several staining solutions and procedures based on the simplified Giemsa technique described by Gray, Wyatt, &amp; Rathbone (1986). The modified Giemsa stain is examined its efficacy and compared with the CLO test using 233\n                      <jats:italic>H.\u00a0pylori<\/jats:italic>\n                      \u2010infected patients with gastric disease.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The modified Giemsa stain is comparable to the traditional one. Statistical analysis indicated that the modified Giemsa stain obtains greater accuracy in\n                      <jats:italic>H.\u00a0pylori<\/jats:italic>\n                      \u2010infected patients with gastritis and ulcer than the CLO test (48.1% vs. 43.7%). Moreover, considering the prognosis of different symptoms of gastric diseases, the modified Giemsa stain has a more accurate prognosis than combination symptoms (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a01.8E\u201005 vs.\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a05.49E\u201005). The modified Giemsa stain is confirmed to be better than CLO test using 233\n                      <jats:italic>H.\u00a0pylori<\/jats:italic>\n                      \u2010infected patients with gastric disease.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The modified Giemsa stain is more simplified and time\u2010saving than traditional Giemsa stain, which is comparable to the traditional one and is confirmed to be better than CLO test using 233\n                      <jats:italic>H.\u00a0pylori<\/jats:italic>\n                      \u2010infected patients with gastric disease. In clinical examination, this modified Giemsa stain can be applied to routine examination and provides quick and accurate diagnosis and prognosis to\n                      <jats:italic>H.\u00a0pylori<\/jats:italic>\n                      \u2010infected patients with gastric diseases.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.23110","type":"journal-article","created":{"date-parts":[[2019,11,16]],"date-time":"2019-11-16T00:11:30Z","timestamp":1573863090000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A time\u2010saving\u2013modified Giemsa stain is a better diagnostic method of\n                    <i>Helicobacter pylori<\/i>\n                    infection compared with the rapid urease test"],"prefix":"10.1002","volume":"34","author":[{"given":"Chi\u2010Chen","family":"Fan","sequence":"first","affiliation":[{"name":"Superintendent Office Mackay Memorial Hospital Taipei Taiwan"},{"name":"Department of Medical Laboratory Science and Biotechnology Yuanpei University of Medical Technology Hsinchu Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chung\u2010Hsing","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Population Health Sciences National Health Research Institutes Zhunan Taiwan"},{"name":"Taiwan Bioinformatics Core National Health Research Institutes Zhunan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Pathology Mackay Memorial Hospital Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting\u2010Yu","family":"Kao","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science and Biotechnology Yuanpei University of Medical Technology Hsinchu Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7145-1878","authenticated-orcid":false,"given":"An Ning","family":"Cheng","sequence":"additional","affiliation":[{"name":"National Institute of Cancer Research National Health Research Institutes Zhunan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0252-0571","authenticated-orcid":false,"given":"Alan Yueh\u2010Luen","family":"Lee","sequence":"additional","affiliation":[{"name":"National Institute of Cancer Research National Health Research Institutes Zhunan Taiwan"},{"name":"Department of Biotechnology Kaohsiung Medical University Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2401-9390","authenticated-orcid":false,"given":"Cheng\u2010Liang","family":"Kuo","sequence":"additional","affiliation":[{"name":"National Institute of Cancer Research National Health Research Institutes Zhunan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.09.004"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0b013e31820fb8f6"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.11.009"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pop.2011.05.001"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365529109098223"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.1984-1990.2002"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1001110"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.17.s2.6.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.2003.00168.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90278-3"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3978\/j.issn.2305-5839.2014.11.03"},{"issue":"4","key":"e_1_2_9_13_1","first-page":"396","article-title":"Comparison of five diagnostic methods for Helicobacter pylori","volume":"5","author":"Khalifehgholi M","year":"2013","journal-title":"Iran J Microbiol"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5107(99)70005-2"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.49.2.107"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000097"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.39.11.1279-a"},{"key":"e_1_2_9_18_1","first-page":"1","article-title":"liver flukes and Helicobacter pylori. 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