{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:32:18Z","timestamp":1789695138270,"version":"4.0.1"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T00:00:00Z","timestamp":1576713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T00:00:00Z","timestamp":1576713600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Children Cancer Fund under the Patronage of HRH Princess Soamsawali and Ramathibodi Foundation","award":["CF_61004"],"award-info":[{"award-number":["CF_61004"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Ethnicity and environmental factors can influence the percentages of lymphocyte subpopulations. This study aimed to assess the percentages of lymphocyte subpopulations according to age in Thai children.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This was a cross\u2010sectional study. The percentages of lymphocyte subpopulations were measured in umbilical cord blood and peripheral blood of healthy Thai children aged 1\u00a0month\u201015\u00a0years. The participants were stratified into five age groups: (a) cord blood; (b) age\u00a0&lt;\u00a02\u00a0years; (c) age 2\u20105\u00a0years; (d) age 5\u201010\u00a0years; and (e) age 10\u201015\u00a0years.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of 182 total samples, 32, 39, 41, 28, and 42 were from cord blood, children aged &lt;2\u00a0years, children aged 2\u20105\u00a0years, children aged 5\u201010\u00a0years, and children aged 10\u201015\u00a0years, respectively. The percentages of most lymphocyte subpopulations including CD8\u00a0+\u00a0T cells, CD19\u00a0+\u00a0cells, \u03b3\u03b4 T cells, double\u2010negative T cells, NK cells, and NK T cells increased significantly with age. Only the CD4+ T\u2010cell percentage decreased in older children. Moderate correlations were observed between age and the percentages of CD4+ T cells, \u03b3\u03b4 T cells, NK cells, NK T cells, and double\u2010negative T cells. Weak correlations were observed between age and the percentages of CD8+ T cells and CD19+ cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study demonstrated age\u2010related changes in the percentages of lymphocyte subpopulations in Thai children, which differed from those described in other countries. Therefore, the establishment of age\u2010specific reference values for lymphocyte subsets in each country is recommended.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.23156","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T11:24:49Z","timestamp":1576754689000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Age\u2010related changes in lymphocyte subpopulations in healthy Thai children"],"prefix":"10.1002","volume":"34","author":[{"given":"Butsabong","family":"Lerkvaleekul","sequence":"first","affiliation":[{"name":"Division of Rheumatology Department of Pediatrics Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nopporn","family":"Apiwattanakul","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease Department of Pediatrics Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chompunuch","family":"Klinmalai","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease Department of Pediatrics Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suradej","family":"Hongeng","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology Department of Pediatrics Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2331-9711","authenticated-orcid":false,"given":"Soamarat","family":"Vilaiyuk","sequence":"additional","affiliation":[{"name":"Division of Rheumatology Department of Pediatrics Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.11.1.168-173.2004"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2003.08.008"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2014.02.010"},{"issue":"5","key":"e_1_2_8_5_1","first-page":"959","article-title":"Peripheral blood lymphocyte subsets in adolescents: a longitudinal analysis from the REACH project","volume":"9","author":"Rudy BJ","year":"2002","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19960315)26:1<8::AID-CYTO2>3.0.CO;2-E"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/sji.12413"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22520"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2003.07.003"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.06.030"},{"issue":"3","key":"e_1_2_8_11_1","first-page":"288","article-title":"Reference ranges for lymphocyte subsets in a defined Malaysian population","volume":"36","author":"Dhaliwal J","year":"1995","journal-title":"Singapore Med J"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.5.5.632-635.1998"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(97)70200-2"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1992.tb12187.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01957900"},{"issue":"3","key":"e_1_2_8_16_1","first-page":"168","article-title":"Distribution of leukocyte and lymphocyte subsets in peripheral blood. 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