{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T13:46:05Z","timestamp":1776087965797,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T00:00:00Z","timestamp":1580256000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T00:00:00Z","timestamp":1580256000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["18JR3RJ429"],"award-info":[{"award-number":["18JR3RJ429"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Our objective was to evaluate the prevalence and different diagnostic methods of breastmilk (BM)\u2010acquired cytomegalovirus (CMV) infection in a pathologically jaundiced cohort.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 400 infants confirmed with pathological jaundice at The People's Hospital of Qingyang City were screened for BM\u2010acquired CMV infection between February 2018 and February 2019. A total of 300 infants were finally enrolled in our study. CMV infection was confirmed by detecting both CMV\u2010DNA in various samples using FQ\u2010PCR and CMV\u2010IgM with chemiluminescence. Clinical and other laboratory data were collected from these infants during their hospitalization or regular visits.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Ninety\u2010eight (32.67%) subjects were confirmed to be BM CMV\u2010DNA\u2013positive, and 18 (18.37%) were diagnosed with a BM\u2010acquired CMV infection. All 18 (100%) infants with a BM\u2010acquired CMV infection were CMV\u2010DNA\u2013positive in urine, while 5 (27.78%) cases and 11 (61.11%) cases were confirmed in plasma and peripheral blood mononuclear cells (PBMCs), respectively. Only 6 (33.33%) infants were CMV\u2010IgM\u2013positive. Birthweight, direct bilirubin, aspartate aminotransferase, and the viral load in BM of the BM\u2010acquired CMV group were higher than those in the non\u2010infected group (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05). Low birthweight and viral load in BM were risk factors for BM\u2010acquired CMV infection. Detecting CMV\u2010DNA in urine samples exhibited better performance than the other methods for screening BM\u2010acquired CMV infections.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study found a high prevalence of BM\u2010acquired CMV infection in jaundiced infants, and detecting CMV\u2010DNA in a urine sample was the most sensitive method for disease screening.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.23199","type":"journal-article","created":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T02:17:42Z","timestamp":1580350662000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["High prevalence of breastmilk\u2010acquired cytomegalovirus infection in jaundiced infants"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8287-2379","authenticated-orcid":false,"given":"Juanjuan","family":"Hou","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Medicine The People Hospital of Qingyang City Qingyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9473-7657","authenticated-orcid":false,"given":"Juan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease The People Hospital of Qingyang City Qingyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0167-3234","authenticated-orcid":false,"given":"Yingfang","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics The People Hospital of Qingyang City Qingyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1898-9817","authenticated-orcid":false,"given":"Hongjun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine The People Hospital of Qingyang City Qingyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8032-8983","authenticated-orcid":false,"given":"Haiyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine The People Hospital of Qingyang City Qingyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1416-1337","authenticated-orcid":false,"given":"Jianmin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine The People Hospital of Qingyang City Qingyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2050-2222","authenticated-orcid":false,"given":"Jiamin","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine The People Hospital of Qingyang City Qingyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0246-9729","authenticated-orcid":false,"given":"Yi","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine The People Hospital of Qingyang City Qingyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4177-6158","authenticated-orcid":false,"given":"Xia","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neonatology The People Hospital of Qingyang City Qingyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6235-6385","authenticated-orcid":false,"given":"Juan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine The People Hospital of Qingyang City Qingyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4103-8155","authenticated-orcid":false,"given":"Xiaoni","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Neonatology The People Hospital of Qingyang City Qingyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0640-3663","authenticated-orcid":false,"given":"Peisong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine The First Affiliated Hospital of Sun Yat\u2010sen University Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,1,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000006007"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1612.12020"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30143-3"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2016.02.042"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4437"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22828"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2018.10.071"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.2174\/1573396315666181126105812"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-4642(18)30139-1"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2017-314063"},{"key":"e_1_2_6_12_1","first-page":"270","article-title":"Clinical and biochemical characteristics of infants with prolonged neonatal jaundice","volume":"24","author":"Siu SL","year":"2018","journal-title":"Hong Kong Med J"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.21858"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2016.11.012"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.114.1.297"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.3906\/sag-1507-161"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03140.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000001835"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14920"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamapediatrics.2014.1360"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2013-0076"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw394"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2014.966080"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24101"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2014.06.002"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2019.05.015"},{"key":"e_1_2_6_27_1","first-page":"53","article-title":"Investigation of congenital CMV infection with the presence of CMV DNA in saliva samples of new born babies","volume":"53","author":"Zeytinoglu A","year":"2019","journal-title":"Mikrobiyol Bul"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2016-013810"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2016.06.024"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0161116"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2011-3552"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.23199","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.23199","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.23199","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.23199","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T12:46:45Z","timestamp":1776084405000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.23199"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,29]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1002\/jcla.23199"],"URL":"https:\/\/doi.org\/10.1002\/jcla.23199","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,29]]},"assertion":[{"value":"2019-09-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23199"}}