{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T13:46:21Z","timestamp":1776087981544,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T00:00:00Z","timestamp":1579824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T00:00:00Z","timestamp":1579824000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Inner Mongolia Science and Technology Program","award":["201502103"],"award-info":[{"award-number":["201502103"]}]},{"DOI":"10.13039\/501100004763","name":"Inner Mongolia natural science foundation","doi-asserted-by":"crossref","award":["2017MS (LH) 0823"],"award-info":[{"award-number":["2017MS (LH) 0823"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Tongliao science and technology planning project","award":["TLKJ2017"],"award-info":[{"award-number":["TLKJ2017"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>GLOBOCAN 2018 latest data show cervical cancer ranks fourth in morbidity and mortality among women. Many genes in cervical lesions differ in sensitivity and specificity. However, the diagnostic molecules for early cervical cancer are not very clear. This paper screens biomarkers for early molecular diagnosis of Mongolian patients with cervical cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Immunohistochemical SP method was used to detect the expression of p16\n                      <jats:sup>INK4a<\/jats:sup>\n                      and Notch1 protein in paraffin sections of 226 Mongolian patients with HPV16\u2010positive cervical lesions after pathological examination, and 100 of them were randomly selected by fluorescence in situ hybridization to detect hTERC gene. The HPV16\u2010binding human cervical cancer SiHa cell line was used to silence the expression of HPV16 E6\/E7 gene by RNA interference, and the expression of p16\n                      <jats:sup>INK4a<\/jats:sup>\n                      , Notch1, and hTERC genes and protein expression levels were detected by RT\u2010PCR and Western blot.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The positive expression rates of p16\n                      <jats:sup>INK4a<\/jats:sup>\n                      , Notch1, and hTERC genes in HPV16\u2010positive cervical cancer, CIN\u2010III, CIN\u2010II, CIN\u2010I, uterine leiomyoma, and chronic cervicitis were significantly different (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05); the positive expression rates of the three genes were also significantly different in the same type of cervical lesions (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05); RNA interference can effectively inhibit HPV16 E6\/E7, p16\n                      <jats:sup>INK4a<\/jats:sup>\n                      and Notch1 gene expression, but has no effect on hTERC gene expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The p16\n                      <jats:sup>INK4a<\/jats:sup>\n                      gene can be used as a biomarker for early screening of cervical cancer, and the hTERC gene can be used to confirm the clinical diagnosis of cervical cancer.\n                    <\/jats:p>\n                  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