{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T09:19:43Z","timestamp":1781774383393,"version":"3.54.5"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T00:00:00Z","timestamp":1578873600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T00:00:00Z","timestamp":1578873600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY15H050001"],"award-info":[{"award-number":["LY15H050001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Rotavirus A and human adenovirus are the two most common causes of infantile diarrhea; thus, it is of great importance to find out a rapid and accurate diagnostic method. This study aimed to evaluate the diagnostic significance of latex agglutination test for detection of rotavirus A and human adenovirus.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A prospective study was conducted on 214 diarrhea children from September 2018 to March 2019 in our hospital. Fresh stool samples were collected for detection of rotavirus A and human adenovirus by latex agglutination test and quantitative reverse transcription polymerase chain reaction (RT\u2010qPCR). Then, the consistency of results detected by these two methods was analyzed\uff0e<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>With performing the latex agglutination test, it was revealed that positive rates for detecting rotavirus A virus and human adenovirus were 23.83% (51\/214) and 25.24% (54\/214), respectively. Meanwhile, results of RT\u2010qPCR showed that positive rates for detecting rotavirus A virus and human adenovirus were 58 (27.10%) and 59 (27.57%), respectively. Using RT\u2010qPCR as the gold standard, the sensitivity and specificity of the latex agglutination test for detecting rotavirus A were 81.03% and 97.44%, and the corresponding values for detecting human adenovirus were 76.27% and 94.19%, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This latex agglutination test showed a satisfactory consistency with RT\u2010qPCR for detecting rotavirus A and human adenovirus. The mentioned commercial assay may be highly appropriate for rapid screening of rotavirus A and human adenovirus.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.23208","type":"journal-article","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T05:49:38Z","timestamp":1578894578000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of a commercial latex agglutination test for detecting rotavirus A and human adenovirus in children's stool specimens"],"prefix":"10.1002","volume":"34","author":[{"given":"Wenqing","family":"Xiang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory The Children's Hospital Zhejiang University School of Medicine National Clinical Research Center For Child Health Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The Children's Hospital Zhejiang University School of Medicine National Clinical Research Center For Child Health Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialu","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Neurology The Children's Hospital Zhejiang University School of Medicine National Clinical Research Center For Child Health Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongqiang","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The Children's Hospital Zhejiang University School of Medicine National Clinical Research Center For Child Health Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7501-5707","authenticated-orcid":false,"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The Children's Hospital Zhejiang University School of Medicine National Clinical Research Center For Child Health Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,13]]},"reference":[{"issue":"3","key":"e_1_2_8_2_1","first-page":"210","article-title":"Prevalence and genotypes of rotavirus A and human adenovirus among hospitalized children with acute gastroenteritis in Fujian, China, 2009\u20132017","volume":"32","author":"Wu BS","year":"2019","journal-title":"Biomed Environ Sci"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.1667"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofy292"},{"issue":"2","key":"e_1_2_8_5_1","first-page":"98","article-title":"Human rotavirus in Iran; molecular epidemiology, genetic diversity and recent updates on vaccine advances","volume":"12","author":"Shadi TN","year":"2019","journal-title":"Spring"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2003"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2027"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/82_2010_16"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-092818-015550"},{"issue":"6","key":"e_1_2_8_10_1","article-title":"The prevalence of rotavirus and adenovirus in the childhood gastroenteritis","volume":"9","author":"Ozsri T","year":"2016","journal-title":"Jundishapur J Microbiol"},{"issue":"4","key":"e_1_2_8_11_1","first-page":"288","article-title":"Development of a quantitative ELISA for detection of rotavirus antigen","volume":"6","author":"Li YF","year":"2016","journal-title":"Chin J Viral Dis"},{"issue":"1","key":"e_1_2_8_12_1","first-page":"81","article-title":"An evaluation of rotavirus and adenovirus antigens by the immunochromatographic method in samples with an initial diagnosis of acute gastroenteritis","volume":"32","author":"Unal N","year":"2016","journal-title":"Acta Medica Mediterranea"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-2712(18)30111-2"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2an35988j"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22230"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.328.7441.702"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000096"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-017-2855-1"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.09.067"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-016-1829-z"},{"issue":"6","key":"e_1_2_8_21_1","first-page":"335","article-title":"Distribution and clinical features of gastrointestinal virus infection in infants with acute diarrhea","volume":"6","author":"Ye HY","year":"2013","journal-title":"Chin J Clin Infect Dis"},{"issue":"2","key":"e_1_2_8_22_1","first-page":"159","article-title":"Epidemiological characteristics of infantile rotavirus diarrhea in Harbin in 2014","volume":"50","author":"Gao J","year":"2016","journal-title":"J Harbin Med Univ"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-4453(83)90527-3"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2013.01.011"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.23208","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.23208","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.23208","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.23208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T12:48:14Z","timestamp":1776084494000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.23208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,13]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10.1002\/jcla.23208"],"URL":"https:\/\/doi.org\/10.1002\/jcla.23208","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,13]]},"assertion":[{"value":"2019-10-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23208"}}