{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:14Z","timestamp":1753869374571,"version":"3.41.2"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T00:00:00Z","timestamp":1581379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Major Infectious Diseases Such as AIDS and Viral Hepatitis Prevention and Control Technology Major Projects","award":["2018ZX10712-001"],"award-info":[{"award-number":["2018ZX10712-001"]}]},{"name":"Zhejiang Medical and Health Science and Technology Plan Project","award":["2018PY077"],"award-info":[{"award-number":["2018PY077"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Candida glabrata<\/jats:italic> is a common pathogen that causes invasive candidiasis. Among non\u2010<jats:italic>albicans Candida<\/jats:italic> infections, <jats:italic>C\u00a0glabrata<\/jats:italic> infections are associated with the highest fatality rates. <jats:italic>Candida glabrata sensu stricto<\/jats:italic>, <jats:italic>Candida nivariensis<\/jats:italic>, and <jats:italic>Candida bracarensis<\/jats:italic> have been identified and together form the <jats:italic>C\u00a0glabrata<\/jats:italic> species complex. It is difficult to detect the two rare species by traditional laboratory methods. This study established a method for the rapid identification of members of the <jats:italic>C<\/jats:italic>\u00a0<jats:italic>glabrata<\/jats:italic> species complex based on high\u2010resolution melting curve (HRM) analysis and evaluated its practical application.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The internal transcribed spacer (ITS) region was used as target gene region to design specific primers. HRM analysis was performed with three subspecies of the <jats:italic>C\u00a0glabrata<\/jats:italic> species complex and negative controls to test its specificity and sensitivity. To evaluate its practical application, the HRM technique was tested with clinical isolates, and the results were compared with the DNA sequencing results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Differences were detected among the melting profiles of the members of the <jats:italic>C<\/jats:italic>\u00a0<jats:italic>glabrata<\/jats:italic> species complex. The negative controls were not amplified, indicating the high specificity of the method. The minimum detection limits of <jats:italic>C\u00a0glabrata sensu stricto<\/jats:italic>, <jats:italic>C\u00a0nivariensis,<\/jats:italic> and <jats:italic>C\u00a0bracarensis<\/jats:italic> were approximately 1\u00a0\u00d7\u00a010<jats:sup>1<\/jats:sup>\u00a0copies\/\u00b5L or less. The results of the HRM analysis of the clinical isolates were consistent with the DNA sequencing results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The HRM method is sensitive and can be used to rapidly identify the members of the <jats:italic>C\u00a0glabrata<\/jats:italic> species complex. The method can allow early and targeted treatment of patients with invasive candidiasis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23226","type":"journal-article","created":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T06:25:21Z","timestamp":1581488721000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Rapid identification of the <i>Candida glabrata<\/i> species complex by high\u2010resolution melting curve analysis"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7721-6537","authenticated-orcid":false,"given":"Shuqian","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Jinhua Municipal Central Hospital Zhejiang China"},{"name":"State Key Laboratory for Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases National Institute for Communicable Disease Control 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