{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T18:31:14Z","timestamp":1780597874826,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,2,10]],"date-time":"2020-02-10T00:00:00Z","timestamp":1581292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Multiple myeloma (MM) is still an incurable hematological malignancy evolved from asymptomatic monoclonal gammopathy of undetermined significance (MGUS). New evidence suggests that circulating microRNAs (miRNAs) can serve as stable diagnostic biomarkers for MM and MUGS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum miRNAs in MM patients, MUGS patients, and healthy controls (HC) were performed by Agilent Bioanalyzer 2100. MicroRNAs in MM detected as promising biomarkers were validated by using quantitative real\u2010time PCR (qRT\u2010PCR). Receiver operator characteristic (ROC) curve and multivariate logistic analysis were used to evaluate the diagnostic value of miRNAs for MM and MUGS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In microarray analysis, the top ten differential expressed miRNAs in MM included miR\u2010134\u20105p, miR\u2010107, miR\u201015a\u20105p, miR\u20105159\u20103p, miR\u20101914\u20103p, miR\u20104723\u20103p, miR\u20105588\u20103p, miR\u20106893\u20103p, miR\u20107106\u20103p, and miR\u20106722\u20105p. Three up\u2010regulated miRNAs (miR\u2010134\u20105p, miR\u2010107, and miR\u201015a\u20105p) were further validated. The elevated expression levels of miR\u2010134\u20105p, miR\u2010107, and miR\u201015a\u20105p in qRT\u2010PCR were increased consistent with microarray analysis. These miRNAs distinguished MM and MUGS from HC significantly. Multivariate logistic analysis showed combination miR\u2010107, miR\u201015a\u20105p with Hb, the AUC was 0.954 (95% CI: 0.890\u20101.000), sensitivity of 91.3%, and specificity of 93.7% for distinguishing MM from MUGS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These data demonstrate that miR\u2010134\u20105p, miR\u2010107, and miR\u201015a\u20105p are potential diagnostic biomarkers in MM and MUGS. Moreover, the combination miR\u2010107 and miR\u201015a\u20105p with Hb can distinguish MM from MUGS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23233","type":"journal-article","created":{"date-parts":[[2020,2,10]],"date-time":"2020-02-10T10:09:49Z","timestamp":1581329389000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Circulating miRNAs as diagnostic biomarkers for multiple myeloma and monoclonal gammopathy of undetermined significance"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5907-9425","authenticated-orcid":false,"given":"Jia","family":"Li","sequence":"first","affiliation":[{"name":"Medical School of Chinese PLA &amp; Medical Laboratory Center The First Medical Center of Chinese PLA General Hospital Beijing China"},{"name":"Clinical Laboratory Medicine Beijing Shijitan Hospital Capital Medical University Beijing China"},{"name":"Beijing Key Laboratory of Urinary 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