{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T04:08:22Z","timestamp":1793678902760,"version":"4.5.1"},"reference-count":17,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T00:00:00Z","timestamp":1582156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672799"],"award-info":[{"award-number":["81672799"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Lymphocyte activation gene 3 (LAG\u20103, also known as CD223) is an immune checkpoint molecule expressed on various types of lymphocytes, and it is mainly involved in maintaining immune homeostasis. However, there are currently no data on LAG\u20103 expression in non\u2013small\u2010cell lung cancer cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human lung cancer cells were cultured using conventional methods. The expression of LAG\u20103 was measured by Western blot and flow cytometry. Between April 2018 and May 2019, we collected 52 surgical specimens of stage I\u2010III non\u2013small\u2010cell lung cancer (NSCLC). Fourteen samples of benign lung tissue lesions were collected as the control group, and the expression levels of LAG\u20103 in the lung cancer cells and tissue samples were measured via immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Western blots showed that LAG\u20103 was expressed in lung cancer cell lines. There was significant difference in the LAG\u20103 expression levels in the NSCLC cells and benign lung tissue (<jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a013.055, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.0003). The LAG\u20103 expression level was significantly associated with the NSCLC clinical stage, and LAG\u20103 expression was significantly higher in stage III patients (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>LAG\u20103 is expressed in NSCLC tumor cells. Furthermore, LAG\u20103 not only is expressed in tumor\u2010infiltrating lymphocytes in NSCLC patients but also is ectopically expressed in tumor cells and associated with TNM stage.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23244","type":"journal-article","created":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T12:19:58Z","timestamp":1582201198000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Ectopic expression of LAG\u20103 in non\u2013small\u2010cell lung cancer cells and its clinical significance"],"prefix":"10.1002","volume":"34","author":[{"given":"Chenglong","family":"Ma","sequence":"first","affiliation":[{"name":"Medical Research Center Changzhou No.2 People's Hospital The Affiliated Hospital of Nanjing Medical University Changzhou China"},{"name":"Department of Oncology The Jiangyin Clinical College of Xuzhou Medical University Jiangyin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Sun","sequence":"additional","affiliation":[{"name":"Medical Research Center Changzhou No.2 People's Hospital The Affiliated Hospital of Nanjing Medical University Changzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Oncology The Jiangyin Clinical College of Xuzhou Medical University Jiangyin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuejun","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathology The Jiangyin Clinical College of Xuzhou Medical University Jiangyin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naifu","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Pathology Changzhou No.2 People's Hospital The Affiliated Hospital of Nanjing Medical University Changzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9567-112X","authenticated-orcid":false,"given":"Chunjian","family":"Qi","sequence":"additional","affiliation":[{"name":"Medical Research Center Changzhou No.2 People's Hospital The Affiliated Hospital of Nanjing Medical University Changzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3775"},{"key":"e_1_2_6_4_1","first-page":"127","article-title":"Tim\u20103, Lag\u20103, and TIGIT","volume":"410","author":"Joller N","year":"2017","journal-title":"Curr Top Microbiol 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