{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T04:44:13Z","timestamp":1764996253957,"version":"3.41.2"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T00:00:00Z","timestamp":1583452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Serum protein electrophoresis (SPE) is a widely used laboratory technique to diagnose patients with multiple myeloma (MM) and other disorders related to serum protein. In patients with MM, abnormal monoclonal protein can be detected by SPE and further characterized using immunofixation electrophoresis (IFE). There are several semi\u2010automated agarose gel\u2010based systems available commercially for SPE and IFE. In this study, we sought to evaluate the analytical performance of fully automated EasyFix G26 (EFG26) and semi\u2010automated HYDRASYS 2 SCAN (H2SCAN) for both SPE and IFE. <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Both instruments were operated according to manufacturer's instructions. Samples used include a commercially available normal control serum (NCS) and patients' specimens. The following were evaluated: precision and comparison studies for SPE, and reproducibility and comparison studies for IFE. Statistical analyses were performed using Microsoft Excel.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For SPE repeatability study, our results showed that EFG26 has higher coefficient of variation (%CV) compared with H2SCAN for both samples except for monoclonal component with %CV of 0.97% and 1.18%, respectively. Similar results were obtained for SPE reproducibility study except for alpha\u20101 (4.16%) and beta (3.13%) fractions for NCS, and beta fractions (5.36%) for monoclonal sample. Subsequently, reproducibility for IFE was 100% for both instruments. Values for correlation coefficients between both instruments ranged from 0.91 to 0.98 for the five classic bands.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Both instruments demonstrated good analytical performance characterized by high precision, reproducibility and correlation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23254","type":"journal-article","created":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T12:50:02Z","timestamp":1583499002000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Performance comparison of EasyFix G26 and HYDRASYS 2 SCAN for the detection of serum monoclonal proteins"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4797-7655","authenticated-orcid":false,"given":"Fatimah Diana","family":"Amin Nordin","sequence":"first","affiliation":[{"name":"Molecular Diagnostics and Protein Unit Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7200-3102","authenticated-orcid":false,"given":"Mohd Khairul Nizam","family":"Mohd Khalid","sequence":"additional","affiliation":[{"name":"Molecular Diagnostics and Protein Unit Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siti Mastura","family":"Abdul Aziz","sequence":"additional","affiliation":[{"name":"Molecular Diagnostics and Protein Unit Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nor Aina","family":"Mohamad Bakri","sequence":"additional","affiliation":[{"name":"Molecular Diagnostics and Protein Unit Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siti Nurwani","family":"Ahmad Ridzuan","sequence":"additional","affiliation":[{"name":"Molecular Diagnostics and Protein Unit Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julaina","family":"Abdul Jalil","sequence":"additional","affiliation":[{"name":"Biochemistry Unit Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anasufiza","family":"Habib","sequence":"additional","affiliation":[{"name":"Biochemistry Unit Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusnita","family":"Yakob","sequence":"additional","affiliation":[{"name":"Molecular Diagnostics and Protein Unit Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.thj.6200312"},{"key":"e_1_2_9_3_1","first-page":"1","article-title":"International myeloma working group recommendations for global myeloma care","author":"Ludwig H","year":"2013","journal-title":"Leukemia"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-10-299529"},{"key":"e_1_2_9_5_1","unstructured":"Sebia Insert Hydragel 4 IF. Acid violet. Ref 4804."},{"volume-title":"Immunology and Serology in Laboratory Medicine","year":"2018","author":"Turgeon ML","key":"e_1_2_9_6_1"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22027"},{"key":"e_1_2_9_8_1","unstructured":"Interlab Insert. Electrophoretic procedure for serum proteins and concentrated urines for: Interlan G26. Kit Ref: SRE 600K\u2010 SRE 601K\u2010 SRE 636K. PK110.12."},{"key":"e_1_2_9_9_1","unstructured":"Sebia Insert. Hydragel 15 Protein (E). Ref 4120."},{"key":"e_1_2_9_10_1","unstructured":"CLSI.User protocol for evaluation of qualitative test performance; approved guideline. Second Edition. CLSI Document EP12\u2010A2. NCCLS USA.2008."},{"key":"e_1_2_9_11_1","unstructured":"Guideline for method verification of quantitative measurement in medical laboratories. Pathology Services. Ministry Of Health Malaysia. [Cited 2018 July 25]. Available from:http:\/\/www.patologi.gov.my\/assets\/policy\/merged.pdf"},{"volume-title":"High\u2010Resolution Electrophoresis and Immunofixation: Techniques and Interpretation","year":"2017","author":"Keren DF","key":"e_1_2_9_12_1"},{"key":"e_1_2_9_13_1","first-page":"23","article-title":"Evaluating assay precision","volume":"29","author":"Chesher D","year":"2008","journal-title":"Clin Biochem Rev"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/32.2.356"},{"key":"e_1_2_9_15_1","first-page":"44","article-title":"Advances in serum protein electrophoresis","volume":"42","author":"Bossuyt X","year":"2006","journal-title":"Adv Clin Chem"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2011.05.022"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.23254","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.23254","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.23254","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.23254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T14:57:04Z","timestamp":1693321024000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.23254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,6]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.1002\/jcla.23254"],"URL":"https:\/\/doi.org\/10.1002\/jcla.23254","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"type":"print","value":"0887-8013"},{"type":"electronic","value":"1098-2825"}],"subject":[],"published":{"date-parts":[[2020,3,6]]},"assertion":[{"value":"2019-02-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23254"}}