{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:37:32Z","timestamp":1788910652496,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T00:00:00Z","timestamp":1583971200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In view of the current difficulty of clinically diagnosing osteoarticular tuberculosis, our aim was to use mass spectrometry to establish diagnostic models and to screen and identify serum proteins which could serve as potential diagnostic biomarkers for early detection of osteoarticular tuberculosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF MS) was used to select an osteoarticular tuberculosis\u2010specific serum peptide profile and establish diagnostic models. Further, liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS) was used to identify potential serum biomarkers that could be used for auxiliary diagnosis of osteoarticular tuberculosis, and then clinical serum samples were used to verify these biomarkers by enzyme\u2010linked immunosorbent assay (ELISA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We established four diagnostic models that can distinguish osteoarticular tuberculosis from rheumatoid arthritis, ankylosing spondylitis, osteoarticular infections, and healthy adults. The models were osteoarticular tuberculosis\u2010rheumatoid arthritis, osteoarticular tuberculosis\u2010ankylosing spondylitis, osteoarticular tuberculosis\u2010osteoarticular infections, and osteoarticular tuberculosis\u2010healthy adult, and their accuracy was 76.78%, 79.02%, 83.77%, and 88.16%, respectively. Next, we selected and identified 18 proteins, including complement factor H\u2010related protein 1 (CFHR1) and complement factor H\u2010related protein 2 (CFHR2), which were upregulated in the tuberculosis group only.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We successfully established four diagnostic models involving osteoarticular tuberculosis, rheumatoid arthritis, ankylosing spondylitis, osteoarticular infections, and healthy adults. Furthermore, we found that CFHR1 and CFHR2 may be two valuable auxiliary diagnostic indicators for osteoarticular tuberculosis. These results provide reference values for rapid and accurate diagnosis of osteoarticular tuberculosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23297","type":"journal-article","created":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T12:13:43Z","timestamp":1584015223000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Screening and identification of serum biomarkers of osteoarticular tuberculosis based on mass spectrometry"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5672-4372","authenticated-orcid":false,"given":"Ximeng","family":"Chen","sequence":"first","affiliation":[{"name":"Center of Clinical Laboratory Medicine The 1st Medical Center of PLA General Hospital Beijing China"},{"name":"School of Laboratory Medicine and Life Science Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingwang","family":"Jia","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Medicine The 1st Medical Center of PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine The 8th Medical Center of PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Wen","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Medicine The 1st Medical Center of PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfei","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine The 8th Medical Center of PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yating","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Medicine Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyun","family":"Ye","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Life Science Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6535-5967","authenticated-orcid":false,"given":"Chengbin","family":"Wang","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Medicine The 1st Medical Center of PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimin","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Life Science Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13323"},{"key":"e_1_2_6_3_1","unstructured":"World Health Organization Global Tuberculosis Report 2018.09.26.http:\/\/www.who.int\/tb\/publications\/global_report\/en\/. 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