{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:50:51Z","timestamp":1790711451732,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T00:00:00Z","timestamp":1593734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81703881"],"award-info":[{"award-number":["81703881"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Chemoresistance posed a barrier to successful treatment of breast cancer (BC), and lncRNA MEG3 has been documented to implicate in BC development. However, whether MEG3 methylation, which led to low MEG3 expression, was relevant to BC progression and chemoresistance remained uncertain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In the aggregate, 374 pairs of tumor tissues and adjacent normal tissues were collected from pathologically confirmed BC patients, and four BC cell lines, including MDA\u2010MB\u2010231, Bcap\u201037, MCF\u20107, and SK\u2010BR\u20103, were purchased. Moreover, methylation\u2010specific polymerase chain reaction (PCR) was adopted to evaluate the methylation status of BC tissues and cell lines, and chemo\u2010tolerance of BC cell lines was assessed by performing MTT assay. Concurrently, transwell assay and scratch assay were carried out to estimate the migratory and invasive capability of BC cell lines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Methylated MEG3, lowly expressed MEG3, large tumor size (\u22652\u00a0cm), advanced TNM grade and lymphatic metastasis were potentially symbolic of poor prognosis among BC patients (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05). Besides, MDA\u2010MB\u2010231 cell line exhibited the strongest resistance against paclitaxel, adriamycin, and vinorelbine (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05), while MCF\u20107 cell line seemed more sensitive against these drugs than any other BC cell line (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05). Furthermore, pcDNA3.1\u2010MEG3 and 5\u2010Aza\u2010dC markedly sensitized MDA\u2010MB\u2010231 and MCF\u20107 cell lines against the drug treatments (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05). Simultaneously, proliferation and metastasis of the BC cell lines were slowed down under the force of pcDNA3.1\u2010MEG3 and 5\u2010Aza\u2010dC (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Preventing methylation of MEG3 might matter in lessening BC chemoresistance, owing to its hindering proliferation and metastasis of BC cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.23369","type":"journal-article","created":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T06:41:08Z","timestamp":1593758468000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Hypermethylation of lncRNA MEG3 impairs chemosensitivity of breast cancer 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