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Worldwide, 96 million infections were estimated in 2010. The dengue virus comprises four distinct serotypes (DENV\u20101, DENV\u20102, DENV\u20103, and DENV\u20104) which belong to the genus Flavivirus. Determining the serotypes during dengue outbreaks is crucial for its effective management in terms of diagnostics improvement and polyvalent vaccine development. The aim of the present study is to determine the prevalence rate of dengue virus serotypes in the samples collected from patients during the 2017 outbreak in Khyber Pakhtunkhwa, Pakistan.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 800 ELISA\u2010positive samples were collected, of which 513 (290 males, 223 females) samples were confirmed positive by PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Out of 513, 25 were found serotype 1 (5%), 196 were serotype 2 (38%), 192 were serotype 3 (37%), 56 were serotype 4 (11%), and 44 (8%) were found to have mix serotypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We can conclude that serotypes 2 and 3 of dengue virus were the predominated serotypes of dengue virus in the 2017 outbreak in Peshawar, capital city of Khyber Pakhtunkhwa, Pakistan.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23371","type":"journal-article","created":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T14:51:46Z","timestamp":1595429506000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Prevalence of dengue virus serotypes in the 2017 outbreak in Peshawar, KP, Pakistan"],"prefix":"10.1002","volume":"34","author":[{"given":"Najeeb Ullah","family":"Khan","sequence":"first","affiliation":[{"name":"Institute of Biotechnology and Genetic Engineering (Health Division) University of Agriculture Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lubna","family":"Danish","sequence":"additional","affiliation":[{"name":"Sulaiman Bin Abdullah Aba Al\u2010Khail Centre for Interdisciplinary in Basic Sciences (SA\u2010CIRBS) International Islamic University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hydayat Ullah","family":"Khan","sequence":"additional","affiliation":[{"name":"Peshawar PCR Lab Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Shah","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Genetic Engineering (Health Division) University of Agriculture Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Ismail","sequence":"additional","affiliation":[{"name":"Department of Zoology Islamia College University Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ijaz","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Biosciences COMSATs University Islamabad Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0353-9151","authenticated-orcid":false,"given":"Arnolfo","family":"Petruzziello","sequence":"additional","affiliation":[{"name":"UOC Clinical Pathology AORN Sant\u2019Anna e San Sebastiano Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rocco","family":"Sabatino","sequence":"additional","affiliation":[{"name":"Unit of Molecular Biology and Viral Oncology Istituto Nazionale Tumori \u2010 IRCCS Fondazione \u201cG. 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