{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T03:21:42Z","timestamp":1776914502429,"version":"3.51.2"},"reference-count":38,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,7,26]],"date-time":"2020-07-26T00:00:00Z","timestamp":1595721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The P\u2010glycoprotein (P\u2010gp) is one of the mechanisms of Imatinib (IM) resistance in chronic myeloid leukemia (CML). P\u2010gp has been identified as an efflux pump involved in releasing of IM outside CML cells. To date, the P\u2010gp involvement in the IM resistance development was not completely understood. Therefore, the present study aimed at measuring the P\u2010gp expression level on lymphocytes from Tunisian patients with CML and correlating this level with a molecular response to IM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>The expression of P\u2010gp on peripheral blood lymphocytes from 59 Tunisian patients with CML (27 IM responder patients vs 32 IM non\u2010responder patients) was evaluated by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>Our finding showed significantly positive expression of P\u2010gp in the lymphocytes from the IM non\u2010responder group when compared to the IM\u2010responder group (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.001). In IM non\u2010responder CML patients, the comparison between CCyR achievers and non\u2010achievers showed a high mean fluorescence intensity (MFI) of P\u2010gp expression in patients who did not achieve their CCyR (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.001). The comparison between patients with primary and secondary resistance to IM showed an increasing MFI value in patients with primary resistance to IM (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.001). Besides, the comparison between nilotinib\u2010treated and dasatinib\u2010treated patients proved a high value of MFI in nilotinib\u2010treated patients (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The overexpression of P\u2010gp on lymphocytes has significantly correlated with the failed molecular response to IM in patients with CML.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23374","type":"journal-article","created":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T03:50:23Z","timestamp":1595821823000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Overexpression of P\u2010glycoprotein and resistance to Imatinib in chronic myeloid leukemia 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