{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:33:52Z","timestamp":1790220832944,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T00:00:00Z","timestamp":1591488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Coronavirus disease 2019 (COVID\u201019) is a pandemic that has rapidly spread worldwide. Increasingly, confirmed patients being discharged according to the current diagnosis and treatment protocols, follow\u2010up of convalescent patients is important to knowing about the outcome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A retrospective study was performed among 98 convalescent patients with COVID\u201019 in a single medical center. The clinical features of patients during their hospitalization and 2\u2010week postdischarge quarantine were collected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the 98 COVID\u201019 convalescent patients, 17 (17.3%) were detected positive severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) nucleic acid during 2\u2010week postdischarge quarantine. The median time from discharge to SARS\u2010CoV\u20102 nucleic acid re\u2010positive was 4\u00a0days (IQR, 3\u20108.5).The median time from symptoms onset to final respiratory SARS\u2010CoV\u20102 detection of negative result was significantly longer in re\u2010positive group (34\u00a0days [IQR, 29.5\u201042.5]) than in non\u2010re\u2010positive group (19\u00a0days [IQR, 16\u201026]). On the other hand, the levels of CD3\u2010CD56\u00a0+\u00a0NK cells during hospitalization and 2\u2010week postdischarge were higher in re\u2010positive group than in non\u2010re\u2010positive group (repeated measures ANOVA, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.018). However, only one case in re\u2010positive group showed exudative lesion recurrence in pulmonary computed tomography (CT) with recurred symptoms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>It is still possible for convalescent patients to show positive for SARS\u2010CoV\u20102 nucleic acid detection, but most of the re\u2010positive patients showed no deterioration in pulmonary CT findings. Continuous quarantine and close follow\u2010up for convalescent patients are necessary to prevent possible relapse and spread of the disease to some extent.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23392","type":"journal-article","created":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T13:31:13Z","timestamp":1591536673000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Clinical features of COVID\u201019 convalescent patients with re\u2010positive nucleic acid detection"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3159-8549","authenticated-orcid":false,"given":"Hui","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Experimental Medical Science HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"},{"name":"Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyun","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province  Ningbo China"},{"name":"Department of Hepatology HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinhua","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Radiology HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiale","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Respiratory HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"},{"name":"Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanhong","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Acute Infection HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyan","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Acute Infection HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Respiratory HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Ying","sequence":"additional","affiliation":[{"name":"Department of Respiratory HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoren","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province  Ningbo China"},{"name":"Department of Hepatology HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of General practice HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanglingzi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"},{"name":"Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"},{"name":"Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjue","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"},{"name":"Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"},{"name":"Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfei","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"},{"name":"Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiachang","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"},{"name":"Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"},{"name":"Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghua","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Cardiology HwaMei Hospital University of Chinese Academy of Sciences  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,7]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"International Committee on Taxonomy of Viruses.Naming the 2019 coronavirus.https:\/\/talk.ictvonline.org\/. Accessed February 25 2020."},{"issue":"1","key":"e_1_2_8_3_1","first-page":"157","article-title":"WHO Declares COVID\u201019 a Pandemic","volume":"91","author":"Cucinotta D","year":"2020","journal-title":"Acta Biomed"},{"key":"e_1_2_8_4_1","unstructured":"World Health Organization.Coronavirus disease (COVID\u201019) outbreak situation.https:\/\/www.who.int\/emergencies\/diseases\/novel-coronavirus-2019. Accessed April 10 2020."},{"issue":"4","key":"e_1_2_8_5_1","first-page":"2012","article-title":"Novel coronavirus 2019\u2010nCoV: prevalence, biological and clinical characteristics comparison with SARS\u2010CoV and MERS\u2010CoV","volume":"24","author":"Meo SA","year":"2020","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2020.35.e79"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa394"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-020-0282-0"},{"key":"e_1_2_8_9_1","unstructured":"The diagnosis and treatment protocols from the national health commission of the People's Republic of China ((trial version sixth).http:\/\/www.nhc.gov.cn\/jkj\/s3577\/202003\/4856d5b0458141fa9f376853224d41d7.shtml. Accessed March 7 2020."},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.1585"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.2131"},{"key":"e_1_2_8_12_1","unstructured":"WHO.Clinical management of severe acute respiratory infection when Novel coronavirus (nCoV) infection is suspected: interim guidance. Jan 28 2020.https:\/\/www.who.int\/internal-publications-detail\/clinical-managementof-severe-acute-respiratory-infection-when-novel-coronavirus-(ncov)-infection-is-suspected. Accessed February 5th 2020."},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30183-5"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hcaa089"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.2783"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2004.02523.x"},{"issue":"4","key":"e_1_2_8_17_1","doi-asserted-by":"crossref","first-page":"2314","DOI":"10.4049\/jimmunol.163.4.2314","article-title":"The human liver contains multiple populations of NK cells, T cells, and CD3+CD56+ natural T cells with distinct cytotoxic activities and Th1, Th2, and Th0 cytokine secretion patterns","volume":"163","author":"Doherty DG","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1537"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-016-0319-y"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003140"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1665-2681(19)31337-7"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.23392","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.23392","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.23392","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T04:31:30Z","timestamp":1693888290000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.23392"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,7]]},"references-count":20,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,7]]}},"alternative-id":["10.1002\/jcla.23392"],"URL":"https:\/\/doi.org\/10.1002\/jcla.23392","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,7]]},"assertion":[{"value":"2020-04-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23392"}}