{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T23:00:19Z","timestamp":1771455619252,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,9,8]],"date-time":"2020-09-08T00:00:00Z","timestamp":1599523200000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Basic research plan of Yunnan Province","award":["2019FE001"],"award-info":[{"award-number":["2019FE001"]}]},{"name":"Basic research plan of Yunnan Province","award":["(-143)"],"award-info":[{"award-number":["(-143)"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In clinical practice, the cases with bacterial infection caused by titanium implants and bacterial biofilm formation on the surface of titanium materials implanted into human body can often be observed. Thus, this study aimed to demonstrate whether the mixed biofilm of <jats:italic>Staphylococcus aureus<\/jats:italic>\/<jats:italic>Escherichia coli<\/jats:italic> can be formed on the surface of titanium material through in vitro experiments and its formation rules.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The titanium plates were put into the well containing <jats:italic>S\u00a0aureus<\/jats:italic> or\/and <jats:italic>E\u00a0coli<\/jats:italic>. Bacterial adhesion and biofilm formation were analyzed by crystal violet, XTT method, confocal laser scanning microscopy, and scanning electron microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results of bacterial adhesion in each group at 6\u201072\u00a0hours showed that the number of bacterial adhesion in each group was increased with the extension of time and reached to the highest level at 72\u00a0hours. Moreover, the biofilm structure in the <jats:italic>S\u00a0aureus<\/jats:italic>\u2010<jats:italic>E\u00a0coli<\/jats:italic> group was significantly more complex than that of the simple <jats:italic>S\u00a0aureus<\/jats:italic> group or <jats:italic>E\u00a0coli<\/jats:italic> group, and the number of bacteria was also significantly increased in the <jats:italic>S\u00a0aureus<\/jats:italic>\u2010<jats:italic>E\u00a0coli<\/jats:italic> group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Those data provide a laboratory basis for the prevention and treatment of mixed infection of subsequent biological materials.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23417","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T07:20:43Z","timestamp":1601018443000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Research on the effect of TiO<sub>2<\/sub> nanotubes coated by gallium nitrate on <i>Staphylococcus aureus<\/i>\u2010<i>Escherichia coli<\/i> biofilm formation"],"prefix":"10.1002","volume":"34","author":[{"given":"Junjie","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Orthopedics The First Affiliated Hospital of Kunming Medical University Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The First Affiliated Hospital of Kunming Medical University Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingquan","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The Third Affiliated Hospital of Kunming Medical University Tumor Hospital of Yunnan Province Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of 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