{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T13:54:24Z","timestamp":1783605264141,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T00:00:00Z","timestamp":1593043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"National Program for Research Development and Innovation II","award":["1196"],"award-info":[{"award-number":["1196"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Fetal nucleated red blood cells (NRBC) from maternal circulation are rare events but can be enriched and used to evaluate the genetics of the fetus. We compared two simplified selection methods of the fetal cells from the maternal blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We isolated fetal cells from maternal blood through double\u2010density gradient centrifugation followed either by magnetic cell selection, based on the paramagnetic proprieties of the NRBC hemoglobin, converted to methemoglobin, or by a positive magnetic\u2010activated cell sorting (MACS) enrichment, using anti\u2010CD71 monoclonal antibodies. Finally, the cells were identified through fluorescence in situ hybridization (FISH) with specific chromosome X and Y probes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We processed 10\u00a0mL of peripheral blood samples from 27 pregnant women with singleton normal male fetuses. Hemoglobin\u2010based enrichment isolated significantly more NRBCs: 29.7\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup> cells than anti\u2010CD71 MACS: 10.1\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup> cells (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001). The FISH analysis found at least one XY cell in 81.5% and 61.5% of cases, respectively, for paramagnetic and anti\u2010CD71 selection. Also, the average number of XY cells identified through paramagnetic selection was 5.09\u00a0\u00b1\u00a02.5, significantly higher than those observed through CD71 sorting: 3.38\u00a0\u00b1\u00a01.7 cells (average\u00a0\u00b1\u00a0SE) (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.03).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The combination of density gradient centrifugation with paramagnetic selection has the advantage of simplicity and achieves a minimal manipulation and treatment of cells. It yields an increased number of NRBCs and FISH confirmed fetal cells, compared to the anti\u2010CD71 sorting.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23420","type":"journal-article","created":{"date-parts":[[2020,6,26]],"date-time":"2020-06-26T05:31:14Z","timestamp":1593149474000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Comparison between paramagnetic and CD71 magnetic activated cell sorting of fetal nucleated red blood cells from the maternal blood"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2345-1077","authenticated-orcid":false,"given":"Dragos","family":"Nemescu","sequence":"first","affiliation":[{"name":"Grigore T Popa University of Medicine and Pharmacy  Iasi 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