{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T15:27:13Z","timestamp":1788794833677,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T00:00:00Z","timestamp":1594771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81802551"],"award-info":[{"award-number":["81802551"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Droplet digital PCR (ddPCR) has been reported to have a superior validity over PCR with amplification\u2010refractory mutation system (ARMS\u2010PCR) for detecting the <jats:italic>BRAF<\/jats:italic> V600E mutation in thyroid nodule fine\u2010needle aspiration (FNA) samples using cytological diagnosis as the reference. However, the added value of ddPCR on surgical decision\u2010making remains to be illustrated when the technique is combined with FNA cytology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 277 consecutive patients with thyroid nodules were subjected to FNA cytology and <jats:italic>BRAF<\/jats:italic> V600E testing with ARMS\u2010PCR. Within this patient cohort, 90 patients underwent surgical intervention with pathological diagnosis available. <jats:italic>BRAF<\/jats:italic> V600E testing with ddPCR was performed retrospectively using FNA frozen DNA specimens. The clinical validity and utility of ddPCR in comparison with ARMS\u2010PCR were compared using surgical pathology as the reference.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, 101 BRAF V600E mutations were detected by ddPCR, including five ARMS negative patients, four of whom were confirmed to have papillary thyroid cancer (PTC) by surgical pathology. Of the 90 patients with surgical pathology, which is considered the gold standard, ddPCR <jats:italic>BRAF<\/jats:italic> V600E testing yielded a sensitivity of 91.3% and specificity of 100% for PTC diagnosis, higher than that of ARMS (sensitivity 83.1%, specificity 100%). However, ddPCR only identified one more candidate patient for surgical intervention than ARMS when the techniques were combined with cytology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study highlighted the superior performance of ddPCR over ARMS in <jats:italic>BRAF<\/jats:italic> V600E detection from thyroid nodule FNA samples. Further studies are needed to evaluate the cost\u2010effectiveness of replacing ARMS\u2010PCR with ddPCR for surgical decision\u2010making.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23458","type":"journal-article","created":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T06:55:14Z","timestamp":1594882514000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Comparison of the Clinical Validity of Droplet Digital PCR to ARMS\u2010PCR for BRAF V600E Mutation Detection in Thyroid Nodules"],"prefix":"10.1002","volume":"34","author":[{"given":"Xiubo","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pathology, Guangzhou First People\u2019s Hospital School of Medicine, South China University of Technology Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Pathology, Guangzhou First 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