{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:49:54Z","timestamp":1787741394943,"version":"build-2784847793"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T00:00:00Z","timestamp":1595894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>As one of the most frequently seen malignancies, hepatocellular carcinoma (HCC) serves as the second largest contributor to malignancy\u2010specific mortality worldwide. MicroRNA\u20101225\u20105p (miR\u20101225) exerts an essential impact on the growth and metastasis of many malignancies. However, the contribution of miR\u2010125 to HCC and the molecular mechanism of cancer cell viability and apoptosis are still unclear. We focused our research on exploring the function and molecular mechanism of miR\u20101225 in regulating HCC cell growth, migration, and invasion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material<\/jats:title><jats:p>Quantitative PCR data showed that miR\u20101225 expression was repressed in HCC cell lines and in the tissues of HCC patients, compared to that in normal human hepatic cells and tissues. Transfection of a miR\u20101225 mimic inhibited cell viability and proliferation as indicated by CCK\u20108 staining and MTT assay. Transwell invasion, wound healing assay, and Western blotting were performed to assess whether miR\u20101225 repressed the metastasis and invasion of HCC cells, and decreased matrix metalloproteinase 9 (MMP9) expression. Further bioinformatic prediction and dual\u2010luciferase reporter assay suggested that miR\u20101225 targeted the 3\u2032\u2010UTR of NF\u03baB p65.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overexpression of p65 protein counteracted the repressive impact of miR\u20101225 on invasion, migration, and proliferation of HCC cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This research provided new evidences that miR\u20101225 inhibits the viability, migration, and invasion of HCC cells by downregulation of p65.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23474","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T10:19:52Z","timestamp":1595931592000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Downregulation of miR\u20101225\u20105p is pivotal for proliferation, invasion, and migration of HCC cells through NF\u03baB regulation"],"prefix":"10.1002","volume":"34","author":[{"given":"Lin","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Oncology Hematology People's Hospital of Linzi District Zibo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiguo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of General Surgery Tianjin Fifth Central Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujing","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Obstetric Area 3 Shandong Qilu Hospital Pingyi Branch (Pingyi County People's Hospital) Linyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangliang","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of General Surgery Tianjin Fifth Central Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6103-5774","authenticated-orcid":false,"given":"Xiangsu","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Hepatolibiary Surgery Jining No.1 People's Hospital Jining 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