{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:25Z","timestamp":1753869385777,"version":"3.41.2"},"reference-count":55,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T00:00:00Z","timestamp":1596758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Deanship of the Scientific Research, Northern Border University","award":["7381-MED-2017-1-8-F"],"award-info":[{"award-number":["7381-MED-2017-1-8-F"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>MicroRNA (miRNA) processing machinery gene variant was associated with several diseases. We aimed to explore for the first time the association of\u00a0machinery gene (<jats:italic>DROSHA<\/jats:italic> rs10719A\/G; <jats:italic>DICER1<\/jats:italic> rs3742330A\/G; <jats:italic>RAN<\/jats:italic> rs14035C\/T; and <jats:italic>XPO5<\/jats:italic> rs11077T\/G) variants with the susceptibility and phenotype of end\u2010stage renal disease (ESRD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>A total of 281 participants (98 ESRD patients and 183 healthy volunteers) were enrolled. Real\u2010Time TaqMan allelic discrimination assay was applied for the genotyping of the specified variants. Multiple logistic regression models, univariate, multivariate, and principal component\u00a0analyses were carried out.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Carrying one <jats:italic>DICER1<\/jats:italic> rs3742330*G allele conferred protection against developing ESRD [heterozygote comparison: OR\u00a0=\u00a00.30, 95% CI\u00a0=\u00a00.15\u20100.62, dominant model: OR\u00a0=\u00a00.35, 95% CI\u00a0=\u00a00.17\u20100.70]. Similarly, for <jats:italic>XPO5<\/jats:italic> rs11077T\/G, homozygote and heterozygote carriers of G variant were less likely to develop ESRD [homozygote comparison: adjusted OR\u00a0=\u00a00.23, 95% CI\u00a0=\u00a00.11\u20100.50, and heterozygote comparison: OR\u00a0=\u00a00.50, 95% CI\u00a0=\u00a00.22\u20100.92, and allelic model: OR\u00a0=\u00a00.46, 95% CI\u00a0=\u00a00.34\u20100.70]. <jats:italic>RAN<\/jats:italic> rs14035*TT subjects were 5 times more likely to develop ESRD while being heterozygote (CT) have twice the risk [homozygote comparison: 5.18, 95% CI\u00a0=\u00a02.28\u201011.8, heterozygote comparison: OR\u00a0=\u00a02.12, 95% CI\u00a0=\u00a01.10\u2010409]. Subgroup analysis also detected <jats:italic>DICER1<\/jats:italic> rs3742330*A, <jats:italic>XPO5<\/jats:italic> rs11077*T, and <jats:italic>RAN<\/jats:italic> rs14035*T as genetic risk determinants for ESRD development in both sex and age categories. Two genotype combinations of <jats:italic>DROSHA\/DICER1\/XPO5<\/jats:italic>\/<jats:italic>RAN<\/jats:italic> were associated with increased susceptibility to ESRD [A\u2010A\u2010T\u2010T: OR\u00a0=\u00a09.49, 95%CI\u00a0=\u00a02.48\u201036.31 (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.001) and G\u2010A\u2010T\u2010T: OR\u00a0=\u00a05.92, 95%CI\u00a0=\u00a01.77\u201019.83 (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.004), respectively].<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Variants and combined genotypes of <jats:italic>DICER1<\/jats:italic> rs3742330, <jats:italic>XPO5<\/jats:italic> rs11077, and <jats:italic>RAN<\/jats:italic> rs14035 might be associated with ESRD development in the study population. Integrating molecular analysis in ESRD risk stratification is warranted.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23520","type":"journal-article","created":{"date-parts":[[2020,8,8]],"date-time":"2020-08-08T06:50:45Z","timestamp":1596869445000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Analysis of microRNA processing machinery gene (<i>DROSHA<\/i>, <i>DICER1, RAN,<\/i> and <i>XPO5<\/i>) variants association with end\u2010stage renal disease"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1252-8403","authenticated-orcid":false,"given":"Manal S.","family":"Fawzy","sequence":"first","affiliation":[{"name":"Department of Biochemistry Faculty of Medicine Northern Border University Arar Saudi Arabia"},{"name":"Department of Medical Biochemistry and Molecular Biology Faculty of Medicine Suez Canal University Ismailia Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3811-0822","authenticated-orcid":false,"given":"Baraah T.","family":"Abu AlSel","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine Northern Border University Arar Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9267-3787","authenticated-orcid":false,"given":"Eman A.","family":"Toraih","sequence":"additional","affiliation":[{"name":"Department of Surgery School of Medicine Tulane University New Orleans LA USA"},{"name":"Genetics Unit Department of Histology and Cell Biology Faculty of Medicine Suez Canal University Ismailia Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/13813455.2018.1499119"},{"issue":"3","key":"e_1_2_6_3_1","first-page":"594","article-title":"Mortality rate of patients with end stage renal disease on regular hemodialysis: a single center study","volume":"22","author":"Elsharif ME","year":"2011","journal-title":"Saudi J Kidney Dis Transpl"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0808700"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2011.189"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1174"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2013.03.002"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00727.2010"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2011.448"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17101712"},{"issue":"11","key":"e_1_2_6_11_1","first-page":"1231","article-title":"MicroRNAs: synthesis, mechanism, function, and recent clinical trials. 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