{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T21:51:10Z","timestamp":1788904270980,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T00:00:00Z","timestamp":1597968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81702057"],"award-info":[{"award-number":["81702057"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Due to the low concentration of androgens in women and the limitation of immunoassays, it remains a challenge to accurately determine the levels of serum androgens in polycystic ovary syndrome (PCOS) patients for clinical laboratories. In this report, a liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS) method was developed and validated for simultaneous quantitation of testosterone (T), androstenedione (A4), dehydroepiandrosterone sulfate (DHEAS), dihydrotestosterone (DHT), and 17\u2010hydroxyprogesterone (17\u2010OHP) that are associated with PCOS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The serum samples were processed by protein precipitation and solid phase extraction before analysis with the in\u2010house developed LC\u2010MS\/MS. The chromatographic separation was achieved with a C18 column, using a linear gradient elution with two mobile phases: 0.02% formic acid in water (phase A) and 0.1% formic acid in methanol (phase B). The separated analytes were detected by positive or negative electrospray ionization mode under multiple reaction monitoring (MRM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The assay for all the five analytes was linear, stable, with imprecision less than 9% and recoveries within \u00b110%. The lower limits of quantification were 0.05, 0.05, 5, 0.025, and 0.025\u00a0ng\/mL for T, A4, DHEAS, DHT, and 17\u2010OHP, respectively. In the receiver operating characteristic curve (ROC) analyses with the PCOS (n\u00a0=\u00a063) and healthy (n\u00a0=\u00a0161) subjects, the AUC of the four\u2010androgen combined was greater than that of any single androgen tested in PCOS diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The LC\u2010MS\/MS method for the four androgens and 17\u2010OHP showed good performance for clinical implementation. More importantly, simultaneous quantitation of the four androgens provided better diagnostic power for PCOS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23539","type":"journal-article","created":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T12:27:06Z","timestamp":1598012826000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Simultaneous quantitation of four androgens and 17\u2010hydroxyprogesterone in polycystic ovarian syndrome patients by LC\u2010MS\/MS"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0968-1864","authenticated-orcid":false,"given":"Zheng","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Beijing Obstetrics and Gynecology Hospital Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Beijing Obstetrics and Gynecology Hospital Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Cong","sequence":"additional","affiliation":[{"name":"Shanghai AB Sciex Analytical Instrument Trading Co., Ltd. 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