{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:13:37Z","timestamp":1789568017628,"version":"build-2803163510"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T00:00:00Z","timestamp":1601251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T00:00:00Z","timestamp":1601251200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Thalassemia is a common inherited hematological disease in tropical and subtropical regions. This study aimed to investigate the mutation spectrum of thalassemia in the Dongguan region of southern China and comprehensively analyze hematologic features of thalassemia carriers with various types of globin mutations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A hematological screening including hematological indices such as mean corpuscular volume (MCV), mean corpuscular hemoglobin content (MCH), and mean corpuscular hemoglobin concentration (MCHC) was conducted in 19\u00a0442 people from Dongguan region, Guangdong province of China. Then, 4891 suspected thalassemia carriers were further investigated by genetic analysis of combined NGS and gap\u2010PCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Totally, 2319 (11.9%) cases were diagnosed as carriers of thalassemia, of which 1483 cases (7.6%) were \u03b1\u2010thalassemia, 741 cases (3.8%) were \u03b2\u2010thalassemia, and 95 cases (0.5%) were co\u2010inheritance of \u03b1\u2010 and \u03b2\u2010thalassemia. In \u03b1\u2010thalassemia carriers, the phenotypic severity increases with the number of nonfunctional \u03b1\u2010globin genes. The patients with \u2013\n                      <jats:sup>SEA<\/jats:sup>\n                      \/\u03b1\n                      <jats:sup>WS<\/jats:sup>\n                      \u03b1 genotype have less severe clinical phenotypes than those with other Hb H diseases. As for \u03b2\u2010thalassemia, the MCV and MCH in both \u03b2\n                      <jats:sup>0<\/jats:sup>\n                      and \u03b2\n                      <jats:sup>+<\/jats:sup>\n                      carriers are markedly reduced.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This is the first comprehensive molecular epidemiological survey and hematological profiling of thalassemia in Dongguan area. This study will be benefit for genetic counseling in the clinic and may help pediatricians to make a correct diagnosis of different types of thalassemia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.23596","type":"journal-article","created":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T13:18:20Z","timestamp":1601299100000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Molecular epidemiological and hematological profile of thalassemia in the Dongguan Region of Guangdong Province, Southern China"],"prefix":"10.1002","volume":"35","author":[{"given":"Qi","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Medical and Molecular Genetics Dongguan Institute of Pediatrics  Dongguan China"},{"name":"Medical Laboratory Dongguan Children's Hospital  Dongguan China"},{"name":"Key Laboratory for Children's Genetics and Infectious Diseases of Dongguan City  Dongguan Guangdong 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