{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:22:24Z","timestamp":1791404544110,"version":"4.3.3"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T00:00:00Z","timestamp":1603152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T00:00:00Z","timestamp":1603152000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Myelodysplastic syndrome (MDS) is a heterogeneous clonal disease originated from hematopoietic stem cells. Epigenetic studies had demonstrated that DNA methylation and histone acetylation were abnormal in MDS. Azacitidine is an effective drug in the treatment of demethylation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>RT\u2010PCR was performed to determine GADD45\u03b3 in 15 MDS clinical samples. Myelodysplastic syndrome cell lines SKM\u20101 and HS\u20105 were transfected with GADD45\u03b3 eukaryotic expression vector and\/or GADD45\u03b3 shRNA interference plasmid, and treated with azacitidine. Proliferation and apoptosis were examined by CCK\u20108 and Western blot analysis to confirm the function role of GADD45\u03b3 and azacitidine. The methylation level of GADD45\u03b3 gene was detected by bisulfite conversion and PCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>This study found that GADD45\u03b3 gene was down\u2010expressed in MDS patients' bone marrow and MDS cell lines, and the down\u2010regulation of GADD45\u03b3 in MDS could inhibit MDS cell apoptosis and promote proliferation. Azacitidine, a demethylation drug, could restore the expression of GADD45\u03b3 in MDS cells and inhibit the proliferation of MDS cells by inducing apoptosis, which was related to prognosis and transformation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study indicated that GADD45\u03b3 was expected to become a new target of MDS\u2010targeted therapy. The findings of this study provided a new direction for the research and development of new MDS clinical drugs, and gave a new idea for the development of MDS demethylation drug to realize precise treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.23597","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T14:46:28Z","timestamp":1603205188000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Azacitidine regulates DNA methylation of GADD45\u03b3 in myelodysplastic syndromes"],"prefix":"10.1002","volume":"35","author":[{"given":"Yanli","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Hematology The First Affiliated Hospital of Bengbu Medical College  Bengbu City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hematology The First 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