{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T19:58:33Z","timestamp":1781812713064,"version":"3.54.5"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T00:00:00Z","timestamp":1603929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T00:00:00Z","timestamp":1603929600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY16H150006"],"award-info":[{"award-number":["LY16H150006"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Melanoma, a relatively common malignancy, has become one of the tumors with the fastest rising incidence in recent years. The purpose of this study was to investigate the effect of Microglial Annexin A3 (ANXA3) on melanoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Serum samples were obtained from 20 patients with melanoma or 20 healthy controls. Kaplan\u2010Meier survival analysis was performed. Transcriptome were used to analyze the correlation between ANXA3 expression and overall survival in patients with melanoma. Human melanoma cell lines WM\u2010115 cells were transfected with ANXA3, si\u2010ANXA3, ANXA3\u00a0+\u00a0si\u2010hypoxia inducible factor\u20101\u03b1 (HIF\u20101\u03b1), si\u2010ANXA3\u00a0+\u00a0HIF\u20101\u03b1, and negative plasmids. Cell proliferation assay, cell invasion assay, and wound healing assay were performed on WM\u2010115 cells. Lactate dehydrogenase (LDH) and caspase\u20103\/9 activities were detected by commercial kits. Western blot and RT\u2010PCR were used to detect the protein and mRNA expression of relation factors.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>ANXA3 expression was up\u2010regulated in patients with melanoma in comparison with healthy controls. Over\u2010expression of ANXA3 promoted cell growth and migration, and reduced cytotoxicity of WM\u2010115 cells. Overall survival (OS) and disease\u2010free survival (DFS) of patients with high ANXA3 expression were both lower than those of patients with low ANXA3 expression. Down\u2010regulation of ANXA3 reduced cell growth and migration, and promoted cytotoxicity of WM\u2010115 cells. ANXA3 induced vascular endothelial growth factor (VEGF) signaling pathway by activation of HIF\u20101\u03b1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In conclusion, our results indicated that ANXA3 promoted cell growth and migration of melanoma via activation of\u00a0HIF\u20101\u03b1\/VEGF signaling pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.23622","type":"journal-article","created":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T04:39:41Z","timestamp":1603946381000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Microglial Annexin A3 promoted the development of melanoma via activation of hypoxia\u2010inducible factor\u20101\u03b1\/vascular endothelial growth factor signaling pathway"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5729-5215","authenticated-orcid":false,"given":"Bin","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Surgery Zhejiang Rehabilitation Medical Center  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Tumor Surgery Zhejiang Cancer Hospital  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Surgery Zhejiang Rehabilitation Medical Center  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfei","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Surgery Zhejiang Rehabilitation Medical Center  Hangzhou 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