{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T09:10:00Z","timestamp":1759741800603,"version":"3.41.2"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T00:00:00Z","timestamp":1607472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Despite the recent improvement in colorectal cancer (CRC) treatment, it still has a poor prognosis with a low survival rate. Genetic and epigenetic mechanisms have proved to play a substantial role in CRC tumorigenesis and progression. According to Gene Ontology and TargetScan analyses, the B\u2010Raf proto\u2010oncogene (<jats:italic>BRAF<\/jats:italic>) gene is one of the microRNA\u201017 (miR\u201017) targets. We aimed to explore the prognostic value of B\u2010Raf protein and<jats:italic>BRAF\/<\/jats:italic>microRNA\u201017 (MIR\u201017) gene expression signature in CRC archived samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>B\u2010Raf protein expression was identified by immunohistochemistry, while gene expression studies were quantified by real\u2010time qPCR in 53 paired archived CRC specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The<jats:italic>BRAF<\/jats:italic>showed higher expressions in CRC specimens relative to non\u2010cancer tissues (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.006). MIR17 expression was inversely and significantly correlated with both B\u2010Raf protein (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.79,<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) and gene expression (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.35,<jats:italic>p<\/jats:italic>\u00a0=\u00a00.010) and showed a significant direct correlation with a high rate of relapse (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.020).<jats:italic>BRAF<\/jats:italic>\/miR\u201017 expression in CRC was associated inversely with tumor size, high grade of colonic carcinoma, lymph node metastasis, and carcinoma subtype. Spearman correlation and Kaplan\u2010Meier survival curve analyses revealed that disease\u2010free survival and overall survival were inversely and significantly correlated with positive B\u2010Raf protein expression (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.31 and \u22120.35,<jats:italic>p<\/jats:italic>\u00a0=\u00a00.023 and 0.011, respectively) and directly correlated with log<jats:italic>BRAF<\/jats:italic>\/MIR17 ratio (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.50 and 0.41,<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001 and\u00a0=\u00a00.003, respectively). Cox hazard regression analysis revealed the<jats:italic>BRAF<\/jats:italic>\/MIR17 ratio could predict both types of patients' survival, among other variables.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>BRAF<\/jats:italic>\/MIR17 ratio could have prognostic utility in patients with CRC. Further larger\u2010scale studies are warranted to confirm this utility.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23679","type":"journal-article","created":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T18:04:16Z","timestamp":1607623456000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Prognostic value of<i>BRAF<\/i>\/MIR\u201017 signature and B\u2010Raf protein expression in patients with colorectal cancer: A pilot study"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8636-0220","authenticated-orcid":false,"given":"Afaf T.","family":"Ibrahiem","sequence":"first","affiliation":[{"name":"Department of Pathology Faculty of Medicine Northern Border University Arar Saudi Arabia"},{"name":"Department of Pathology Faculty of Medicine Mansoura University Mansoura 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