{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:41Z","timestamp":1753869401349,"version":"3.41.2"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Otitis media (OM) is a middle ear inflammatory complex disorder involving genetic and environmental factors. It onsets during childhood and often recurs and perplexes in genetically susceptible patients. Previously, murine models had shown the association of <jats:italic>ISL LIM<\/jats:italic> homeobox 1 (<jats:italic>ISL1<\/jats:italic>) gene with otitis media with effusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the association of <jats:italic>ISL1<\/jats:italic> genetic variants with otitis media.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and methods<\/jats:title><jats:p>A total of 285 cases and 277 controls were recruited for the study. The entire coding region of <jats:italic>ISL1<\/jats:italic> gene was genotyped using Sanger sequencing or single\u2010strand conformation polymorphism methods. Genotype, haplotype, <jats:italic>in silico<\/jats:italic> analysis, and linkage disequilibrium analysis were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The variants rs2303751 (c.504A&gt;G) and rs121913540 (c.513G&gt;A) were associated with OM, and the OR (95%CI) was 0.74 (0.57\u20130.95) and 0.43 (0.20\u20130.91), respectively. Besides, the rs2303751 AA genotype was associated with elevated eosinophil numbers in OM when compared to controls. The 5 SNP haplotype analysis of SNPs c.\u2010492A&gt;G, c.504A&gt;G, c.513G&gt;A, c.576C&gt;T, and c.*651A&gt;T revealed A\u2010A\u2010G\u2010C\u2010A to be a risk haplotype in females whereas the 3 SNP haplotype analysis of SNPs c.504A&gt;G, c.513G&gt;A, and c.567C&gt;T suggested G\u2010A\u2010C as protective and A\u2010G\u2010C to be a risk haplotype for otitis media.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Ours is the first report which shows a significant association of <jats:italic>ISL1<\/jats:italic> variants (rs2303751 and rs121913540) with hearing\u2010related disorder like otitis media in humans. These results implicate the possible role of <jats:italic>ISL1<\/jats:italic> gene in the etiopathology of otitis media. The replication of the study in other ethnic populations may strengthen our findings.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23702","type":"journal-article","created":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T20:25:04Z","timestamp":1611260704000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Association of <i>ISL1<\/i> polymorphisms and eosinophilic levels among otitis media patients"],"prefix":"10.1002","volume":"35","author":[{"given":"Abhishek","family":"Kondyarpu","sequence":"first","affiliation":[{"name":"Institute of Life Sciences Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chinmay Sundar","family":"Ray","sequence":"additional","affiliation":[{"name":"Department of Ear, Nose, and Throat (ENT) Shrirama Chandra Bhanja (SCB) Medical College &amp; Hospital Cuttack India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khirod 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