{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T05:03:40Z","timestamp":1776747820264,"version":"3.51.2"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,2,5]],"date-time":"2021-02-05T00:00:00Z","timestamp":1612483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LGF18H160021"],"award-info":[{"award-number":["LGF18H160021"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012175","name":"Zhejiang Traditional Chinese Medicine Administration","doi-asserted-by":"publisher","award":["2017ZA046"],"award-info":[{"award-number":["2017ZA046"]}],"id":[{"id":"10.13039\/501100012175","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Myasthenia gravis (MG) is an autoimmune disease mediated by acetylcholine receptor antibodies. Exosomes were shown to be involved in the immune modulation and autoimmune diseases. However, the expression and function of exosomal long noncoding RNAs (lncRNAs) in MG are still unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We conducted high\u2010throughput sequencing to detect the lncRNA profiles of serum exosomes in 6 MG patients (2 grade I, 2 grade IIa, and 2 grade IIb) and 6 healthy controls (HC). Then, differentially expressed (DE) lncRNAs with the greatest difference between the MG and HC groups were selected for further quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) validation in additional 30 MG patients and 10 HC. The DE lncRNAs were used to construct the coding\/noncoding network and perform enrichment analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified 378 significantly upregulated and 348 significantly downregulated lncRNAs in MG patients compared with HC. The top 5 lncRNAs (NR_104677.1, ENST00000583253.1, NR_046098.1, NR_022008.1, and ENST00000581362.1) were validated and shown to be significantly increased in the serum exosome of MG, and the expression level of NR_046098.1 significantly increased with the MG grading. Enrichment analysis showed that DE genes mainly participated in the basic biological regulation of MG and immune\u2010related pathways, such as autoimmune thyroid disease pathway and T\u2010cell receptor signaling pathway. A specific lncRNA\u2010miRNA\u2010mRNA regulatory network associated with the 5 lncRNAs, 14 MG\u2010related miRNAs and 30 mRNAs was constructed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We conducted a comprehensive analysis of exosomal lncRNAs to reveal potential biomarkers for the MG diagnosis and severity assessment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23722","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T05:40:29Z","timestamp":1612762829000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Expression profiling and bioinformatics analysis of exosomal long noncoding RNAs in patients with myasthenia gravis by RNA sequencing"],"prefix":"10.1002","volume":"35","author":[{"given":"Wei","family":"Lu","sequence":"first","affiliation":[{"name":"Clinical Laboratory The First Affiliated Hospital of Zhejiang Chinese Medical University  Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Lu","sequence":"additional","affiliation":[{"name":"Clinical Laboratory The First Affiliated Hospital of Zhejiang Chinese Medical University  Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun\u2010Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Clinical Laboratory The First Affiliated Hospital of Zhejiang Chinese Medical University  Hangzhou Zhejiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0956-8869","authenticated-orcid":false,"given":"Ting\u2010Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Clinical Laboratory The First Affiliated Hospital of Zhejiang Chinese Medical University  Hangzhou Zhejiang Province 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