{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T02:34:27Z","timestamp":1772505267903,"version":"3.50.1"},"reference-count":10,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T00:00:00Z","timestamp":1615766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>An immunochromatography technology (ICT) rapid diagnostic test, the <jats:italic>Toxoplasma<\/jats:italic> ICT IgG\u2010IgM<jats:sup>\u00ae<\/jats:sup>, was recently developed. Our aim was to study its contribution to establish accurately the <jats:italic>Toxoplasma<\/jats:italic> immune status in Tunisian pregnant women using Western blot (WB) Toxo II IgG<jats:sup>\u00ae<\/jats:sup> as a reference technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010nine sera were selected for the study from among 2,615 which were already tested by IgG and IgM ELISA. They displayed equivocal IgG titres (4.4\u20139\u00a0IU\/ml) in absence of IgM (19\u00a0sera) or IgM anti\u2010<jats:italic>Toxoplasma<\/jats:italic> antibodies in absence of IgG (titre &lt;4.4\u00a0IU\/ml) (20 sera). All these sera were additionally tested by WB Toxo II IgG<jats:sup>\u00ae<\/jats:sup>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunochromatography technology Sensitivity in the detection either of low IgG titres in absence of IgM or of specific anti\u2010<jats:italic>Toxoplasma<\/jats:italic> IgM was 100%. Only one serum with equivocal IgG titre by ELISA and negative with Toxo II IgG<jats:sup>\u00ae<\/jats:sup> test revealed positive in ICT. However, this serum showed a P30 band in WB analysis. On the other hand, three sera positive in ELISA IgM and negative in ELISA IgG revealed positive in ICT and negative in WB Toxo II IgG<jats:sup>\u00ae<\/jats:sup>, the reference test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Results confirm the high sensitivity of <jats:italic>Toxoplasma<\/jats:italic> ICT IgG\u2010IgM<jats:sup>\u00ae<\/jats:sup> in detecting both specific anti\u2010<jats:italic>Toxoplasma<\/jats:italic> IgG and IgM, and highlight the usefulness of this rapid test as a first or second\u2010line <jats:italic>Toxoplasma<\/jats:italic> serological test in pregnant women.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23749","type":"journal-article","created":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T10:16:36Z","timestamp":1615803396000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Contribution of the <i>Toxoplasma<\/i> ICT IgG IgM<sup>\u00ae<\/sup> test in determining the immune status of pregnant women against toxoplasmosis"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4282-1320","authenticated-orcid":false,"given":"Ben\u2010Abdallah","family":"Rym","sequence":"first","affiliation":[{"name":"Department of Parasitology Pasteur Institute of Tunis Tunis Tunisia"},{"name":"Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR 11IPT\u201006 Pasteur Institute of Tunis University Tunis El\u2010Manar Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalboussi","family":"Yasmine","sequence":"additional","affiliation":[{"name":"Department of Parasitology Pasteur Institute of Tunis Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bellali","family":"Hedia","sequence":"additional","affiliation":[{"name":"Department of Epidemiology Abderrahmane Mami Hospital Ariana Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Issaoui","family":"Nesrine","sequence":"additional","affiliation":[{"name":"Department of Parasitology Pasteur Institute of Tunis Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Souissi","family":"Olfa","sequence":"additional","affiliation":[{"name":"Department of Parasitology Pasteur Institute of Tunis Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maatoug","family":"Rania","sequence":"additional","affiliation":[{"name":"Department of Parasitology Pasteur Institute of Tunis Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aoun","family":"Karim","sequence":"additional","affiliation":[{"name":"Department of Parasitology Pasteur Institute of Tunis Tunis Tunisia"},{"name":"Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR 11IPT\u201006 Pasteur Institute of Tunis University Tunis El\u2010Manar Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bouratbine","family":"A\u00efda","sequence":"additional","affiliation":[{"name":"Department of Parasitology Pasteur Institute of Tunis Tunis Tunisia"},{"name":"Research Laboratory Medical Parasitology, Biotechnology and Biomolecule LR 11IPT\u201006 Pasteur Institute of Tunis University Tunis El\u2010Manar Tunis 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