{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T16:52:20Z","timestamp":1778777540634,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T00:00:00Z","timestamp":1615766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>To establish a time\u2010resolved fluorescence immunoassay of interleukin (IL)\u201018 (IL\u201018\u2010TRFIA) and detect its concentration in different liver disease serum samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The IL\u201018 coating antibody and the Eu<jats:sup>3+<\/jats:sup>\u2010labeled detection antibody were used for the IL\u201018\u2010TRFIA to detect serum IL\u201018 concentration in patients with liver cancer, hepatitis B, hepatitis C, autoimmune hepatitis, fatty liver disease, and healthy controls. The double\u2010antibody sandwich method was used and methodological evaluation was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The average intra\u2010 and inter\u2010assay coefficient of variation for IL\u201018\u2010TRFIA was 4.80% and 5.90%, respectively. The average recovery rate was 106.19\u00a0\u00b1\u00a03.44%. The sensitivity (10.96\u00a0pg\/mL) was higher than that obtained using the ELISA method (62.5\u00a0pg\/mL). The detection range was 10.96\u20131000\u00a0pg\/mL. IL\u20106 and galectin\u20103 did not cross\u2010react with IL\u201018\u2010TRFIA. The serum concentration of IL\u201018 was (776.99; 653.48\u2013952.39\u00a0pg\/mL) in hepatitis C, (911; 775.55\u20131130.03\u00a0pg\/mL) in fatty liver, (1048.88; 730.04\u20131185.10\u00a0pg\/mL) in liver cancer, and (949.12; 723.70\u20131160.28\u00a0pg\/mL) in hepatitis B. Moreover, IL\u201018 serum levels were significantly higher in patients than the healthy controls (483.09; 402.52\u2013599.70\/mL) (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.0001). Autoimmune hepatitis with a serum IL\u201018 concentration of 571.62; 502.47\u2013730.31\u00a0pg\/mL was not significantly different from the healthy controls (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We established a highly sensitive IL\u201018\u2010TRFIA method that successfully detected serum IL\u201018 concentrations in different liver diseases. Furthermore, IL\u201018 serum concentration was higher in patients with liver cancer, hepatitis C, hepatitis B, and fatty liver disease compared to healthy controls.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23758","type":"journal-article","created":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T10:14:36Z","timestamp":1615803276000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Establishment of interleukin\u201018 time\u2010resolved fluorescence immunoassay and its preliminary application in liver disease"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7379-7258","authenticated-orcid":false,"given":"Li","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Life Sciences and Medicine Zhejiang Sci\u2010Tech University Hangzhou 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