{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T22:48:27Z","timestamp":1776379707502,"version":"3.51.2"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T00:00:00Z","timestamp":1620000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Marburg virus (MARV) and Ebola virus (EBOV) are acute infections with high case fatality rates. It is of great significance for epidemic monitoring and prevention and control of infectious diseases by the development of a rapid, specific, and sensitive quantitative PCR method to detect two pathogens simultaneously.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Primers and TaqMan probes were designed according to highly conserved sequences of these viruses. Sensitivity, specificity, linear range, limit of detection, and the effects of hemolysis and lipid on real\u2010time qPCR were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The linearity of the curve allowed quantification of nucleic acid concentrations in range from 10<jats:sup>3<\/jats:sup> to 10<jats:sup>9<\/jats:sup>\u00a0copies\/ml per reaction (MARV and EBOV). The limit of detection of EBOV was 40\u00a0copies\/ml, and MARV was 100\u00a0copies\/ml. It has no cross\u2010reaction with other pathogens such as hepatitis b virus (HBV), hepatitis c virus (HCV), human papillomavirus (HPV), Epstein\u2010Barr virus (EBV), herpes simplex virus (HSV), cytomegalovirus (CMV), and human immunodeficiency virus (HIV). Repeatability analysis of the two viruses showed that their coefficient of variation (CV) was less than 5.0%. The above results indicated that fluorescence quantitative PCR could detect EBOV and MARV sensitively and specifically.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The TaqMan probe\u2010based multiplex fluorescence quantitative PCR assays could detect EBOV and MARV sensitively specifically and simultaneously.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23786","type":"journal-article","created":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T16:02:01Z","timestamp":1620057721000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Simultaneous detection of Marburg virus and Ebola virus with TaqMan\u2010based multiplex real\u2010time PCR method"],"prefix":"10.1002","volume":"35","author":[{"given":"Zhikang","family":"Yu","sequence":"first","affiliation":[{"name":"Center for Precision Medicine Meizhou People's Hospital (Huangtang Hospital) Meizhou Academy of Medical Sciences Meizhou 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