{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T22:24:33Z","timestamp":1773527073194,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T00:00:00Z","timestamp":1620000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Stroke is a devastating complication of sickle cell anemia (SCA) and can be predicted through abnormally high cerebral blood flow velocity using transcranial Doppler Ultrasonography (TCD). The evidence on the role of alpha\u2010thalassemia and glucose\u20106\u2010phosphate dehydrogenase (<jats:italic>G6PD<\/jats:italic>) deficiency in the development of stroke in children with SCA is conflicting. Thus, this study investigated the association of alpha\u2010thalassemia and G6PD(A<jats:sup>\u2212<\/jats:sup>) variant with abnormal TCD velocities among Nigerian children with SCA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>One hundred and forty\u2010one children with SCA were recruited: 72 children presented with normal TCD (defined as the time\u2010averaged mean of the maximum velocity:\u00a0&lt;\u00a0170\u00a0cm\/s) and 69 children with abnormal TCD (TAMMV\u00a0\u2265\u00a0200\u00a0cm\/s). Alpha\u2010thalassemia (the \u03b1\u20103.7 globin gene deletion) was determined by multiplex gap\u2010PCR, while G6PD polymorphisms (202G\u00a0&gt;\u00a0A and 376A\u00a0&gt;\u00a0G) were genotyped using restriction fragment length polymorphism\u2014polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The frequency of \u03b1\u2010thalassemia trait in the children with normal TCD was higher than those with abnormal TCD: 38\/72 (52.8%) [\u03b1\u2010\/ \u03b1 \u03b1: 41.7%, \u03b1 \u2010\/ \u03b1 \u2010: 11.1%] versus 21\/69 (30.4%) [\u03b1\u2010\/ \u03b1 \u03b1: 27.5%, \u03b1 \u2010\/ \u03b1 \u2010: 2.9%], and the odds of abnormal TCD were reduced in the presence of the \u03b1\u2010thalassemia trait [Odds Ratio: 0.39, 95% confidence interval: 0.20\u20130.78, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.007]. However, the frequencies of G6PDA<jats:sup>\u2212<\/jats:sup> variant in children with abnormal and normal TCD were similar (11.6% vs. 15.3%, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.522).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study reveals the protective role of \u03b1\u2010thalassemia against the risk of abnormal TCD in Nigerian children with SCA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23802","type":"journal-article","created":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T11:14:43Z","timestamp":1620040483000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Association of alpha\u2010thalassemia and Glucose\u20106\u2010Phosphate Dehydrogenase deficiency with transcranial Doppler ultrasonography in Nigerian children with sickle cell anemia"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2774-650X","authenticated-orcid":false,"given":"Oyesola Oyewole","family":"Ojewunmi","sequence":"first","affiliation":[{"name":"Sickle Cell Foundation Nigeria  Surulere, Lagos Nigeria"},{"name":"School of Cancer and Pharmaceutical Sciences King's College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Titilope Adenike","family":"Adeyemo","sequence":"additional","affiliation":[{"name":"Department of Haematology &amp; Blood Transfusion College of Medicine University of Lagos  Lagos Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajoke Idayat","family":"Oyetunji","sequence":"additional","affiliation":[{"name":"Sickle Cell Foundation Nigeria  Surulere, Lagos Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yewande","family":"Benn","sequence":"additional","affiliation":[{"name":"Sickle Cell Foundation Nigeria  Surulere, Lagos Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mfoniso Godwin","family":"Ekpo","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Afe Babalola University  Ado\u2010Ekiti Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bamidele Abiodun","family":"Iwalokun","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biotechnology Nigerian Institute of Medical Research  Lagos Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12300"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-012-1204-9"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.25216"},{"issue":"1","key":"e_1_2_8_5_1","first-page":"288","article-title":"Cerebrovascular accidents in sickle cell disease: rates 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