{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T20:09:21Z","timestamp":1790453361215,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T00:00:00Z","timestamp":1624233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Despite medical advances, central nervous system (CNS) diseases put a pressure on the health care system. A number of risk factors, especially infectious agents can accelerate the progression of meningitis. As viruses probably account for most cases of meningitis, the diagnosis of them can reduce antibiotic prescriptions. Among various types of infectious diseases, the relationship between two important virus families, including Picornaviridae and Herpesviridae, and meningitis has attracted attraction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, one hundred and two samples were collected from patients who experienced symptoms, such as the loss of consciousness, seizures, muscle weakness, fever, headache, rash, and severe dementia, between November 2018 and September 2019. After RNA and DNA extraction, the prevalence of Enterovirus (EV), Cytomegalovirus (CMV), Epstein\u2013Barr virus (EBV), Herpes simplex virus type 1 (HSV\u20101), Herpes simplex virus type 2 (HSV\u20102), and Varicella zoster virus (VZV) was evaluated using PCR, multiplex PCR, and nested PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Results indicated that there were two VZV DNA\u2010positive specimens, while six and five samples were infected with HSV\u20101 and EBV, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We reported that the prevalence of EBV, HSV\u20101, and VZV in patients, suffering from meningitis cannot be ignored; however, further investigation is needed.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23836","type":"journal-article","created":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T07:30:53Z","timestamp":1624260653000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Detection of <i>Enterovirus<\/i>, <i>Herpes Simplex<\/i>, <i>Varicella Zoster<\/i>, <i>Epstein\u2010Barr<\/i> and <i>Cytomegalovirus<\/i> in cerebrospinal fluid in meningitis patients in Iran"],"prefix":"10.1002","volume":"35","author":[{"given":"Shaian","family":"Tavakolian","sequence":"first","affiliation":[{"name":"Infectious Diseases and Tropical Medicine Research Center Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Goudarzi","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Medicine Shahid Beheshti University of Medical Sciences  Tehran 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