{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:07:19Z","timestamp":1790172439260,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T00:00:00Z","timestamp":1626048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T00:00:00Z","timestamp":1626048000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2017JJA10152"],"award-info":[{"award-number":["2017JJA10152"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This study aimed to evaluate the urate\u2010lowering effects of Yi\u2010Suan\u2010Cha and explore its underlying mechanisms in experimental hyperuricemia induced in rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Forty\u2010eight male SD rats were randomly allocated into normal control, model, allopurinol, benzbromarone, low\u2010dose Yi\u2010Suan\u2010Cha (0.2\u00a0g\/ml), and high\u2010dose Yi\u2010Suan\u2010Cha (0.4\u00a0g\/ml) groups (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a08 rats per group). Rat models of hyperuricemia were established through intragastric administration of adenine 25\u00a0mg\/kg\u00a0+\u00a0potassium oxalate 300\u00a0mg\/kg for 3\u00a0weeks. After the last administration, serum uric acid, creatinine, and urea nitrogen levels were measured. Renal histopathology was observed by hematoxylin\u2010eosin staining. Xanthine oxidase level in serum and liver homogenates was measured by ELISA. The protein and mRNA expression of URAT1, ABCG2, OAT1, and GLUT9 in the kidney was detected by Western blotting and RT\u2010PCR, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The serum uric acid levels were significantly lowered in all medication groups than in the model group. The benzbromarone and both Yi\u2010Suan\u2010Cha groups showed clear kidney structures with no obvious abnormalities. Compared with the normal control group, the model group showed increased URAT1\/GLUT9 protein expression and decreased ABCG2\/OAT1 protein expression. Compared with the model group, both Yi\u2010Suan\u2010Cha groups showed decreased URAT1\/GLUT9 protein expression and increased ABCG2\/OAT1 protein expression. Compared with that in the normal control group, URAT1\/GLUT9 mRNA expression increased in the model group. Compared with the model group, the low\u2010dose and high\u2010dose Yi\u2010Suan\u2010Cha groups showed decreased URAT1\/GLUT9 mRNA expression and increased ABCG2\/OAT1 mRNA expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Yi\u2010Suan\u2010Cha may lower uric acid level by downregulating URAT1\/GLUT9 expression and upregulating ABCG2\/OAT1 expression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.23859","type":"journal-article","created":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T05:44:36Z","timestamp":1626068676000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Ameliorative effect and mechanism of Yi\u2010Suan\u2010Cha against hyperuricemia in rats"],"prefix":"10.1002","volume":"35","author":[{"given":"Yuanyuan","family":"Qin","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Guangxi Medical University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Guangxi Medical University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Tao","sequence":"additional","affiliation":[{"name":"Guangxi Medical College Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Guangxi Medical University Nanning 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