{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:06:39Z","timestamp":1790172399304,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T00:00:00Z","timestamp":1623801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T00:00:00Z","timestamp":1623801600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pooling of samples for SARS\u2010CoV\u20102 testing in low\u2010prevalence settings has been used as an effective strategy to expand testing capacity and mitigate challenges with the shortage of supplies. We evaluated two automated molecular test systems for the detection of SARS\u2010CoV\u20102 RNA in pooled specimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Pooled nasopharyngeal and saliva specimens were tested by Qiagen QIAstat\u2010Dx Respiratory SARS\u2010CoV\u20102 Panel (QIAstat) or Cepheid Xpert Xpress SARS\u2010CoV\u20102 (Xpert), and the results were compared to that of standard RT\u2010qPCR tests without pooling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In nasopharyngeal specimens, the sensitivity\/specificity of the pool testing approach, with 5 and 10 specimens per pool, were 77%\/100% (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0105) and 74.1%\/100% (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0260) by QIAstat, and 97.1%\/100% (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0250) and 100%\/99.5% (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0200) by Xpert, respectively. Pool testing of saliva (10 specimens per pool;\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0150) by Xpert resulted in 87.5% sensitivity and 99.3% specificity compared to individual tests. Pool size of 5 or 10 specimens did not significantly affect the difference of RT\u2010qPCR cycle threshold (C\n                      <jats:sub>T<\/jats:sub>\n                      ) from standard testing. RT\u2010qPCR C\n                      <jats:sub>T<\/jats:sub>\n                      values obtained with pool testing by both QIAstat and Xpert were positively correlated with that of individual testing (Pearson's correlation coefficient\n                      <jats:italic>r<\/jats:italic>\n                      \u00a0=\u00a00.85 to 0.99,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). However, the C\n                      <jats:sub>T<\/jats:sub>\n                      values from Xpert were significantly stronger (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01, paired t test) than that of QIAstat in a subset of SARS\u2010CoV\u20102 positive specimens, with mean differences of \u22124.3\u00a0\u00b1\u00a02.43 and \u22124.6\u00a0\u00b1\u00a02 for individual and pooled tests, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our results suggest that Xpert SARS\u2010CoV\u20102 can be utilized for pooled sample testing for COVID\u201019 screening in low\u2010prevalence settings providing significant cost savings and improving throughput without affecting test quality.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.23876","type":"journal-article","created":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T05:41:37Z","timestamp":1623822097000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of automated molecular tests for the detection of SARS\u2010CoV\u20102 in pooled nasopharyngeal and saliva specimens"],"prefix":"10.1002","volume":"35","author":[{"given":"Hamad","family":"Al\u2010Hail","sequence":"first","affiliation":[{"name":"Sidra Medicine Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faheem","family":"Mirza","sequence":"additional","affiliation":[{"name":"Sidra Medicine Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaa","family":"Al Hashemi","sequence":"additional","affiliation":[{"name":"Sidra Medicine Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muneera Naseer","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Sidra Medicine Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Sidra Medicine Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Tang","sequence":"additional","affiliation":[{"name":"Sidra Medicine Doha Qatar"},{"name":"Weill Cornell Medical College in Qatar Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4658-7949","authenticated-orcid":false,"given":"Mohammad Rubayet","family":"Hasan","sequence":"additional","affiliation":[{"name":"Sidra Medicine Doha Qatar"},{"name":"Weill Cornell Medical College in Qatar Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2020.105955"},{"key":"e_1_2_7_3_1","unstructured":"CDC.Information for Laboratories about Coronavirus (COVID\u201019) 2020.https:\/\/www.cdc.gov\/coronavirus\/2019\u2010nCoV\/lab\/index.html. 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Accessed 23 December 2020."}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.23876","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.23876","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.23876","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:38:47Z","timestamp":1790170727000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.23876"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,16]]},"references-count":20,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1002\/jcla.23876"],"URL":"https:\/\/doi.org\/10.1002\/jcla.23876","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,16]]},"assertion":[{"value":"2021-05-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23876"}}