{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T07:20:54Z","timestamp":1776583254596,"version":"3.51.2"},"reference-count":18,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,7,10]],"date-time":"2021-07-10T00:00:00Z","timestamp":1625875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Salmonella Wandsworth<\/jats:italic> is a rare serotype of <jats:italic>Salmonella<\/jats:italic>. This study analyzed the genotyping, genome structure, and molecular biological functions of <jats:italic>Salmonella Wandsworth<\/jats:italic> based on the results of multilocus sequence typing and next\u2010generation sequencing genome assembly analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serological typing was performed using the slide\u2010agglutination method. The micro broth dilution method was used to test antibiotic susceptibility. Multilocus sequence typing (MLST) was used to perform the homology analysis, while the second\u2010generation sequencing genome analysis was used to analyze the whole genome of the bacteria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Salmonella Wandsworth<\/jats:italic> is Group Q <jats:italic>Salmonella<\/jats:italic>. The MLST of this strain was ST1498. <jats:italic>Salmonella Wandsworth<\/jats:italic> was sensitive to antibiotics, such as ceftriaxone, imipenem, chloramphenicol, and colistin, but was resistant to ampicillin, cefalotin, gentamicin, and ciprofloxacin. The second\u2010generation sequencing results showed that the genome sequence length of the bacteria was 5109457bp. Annotated COG library analysis generated 3,746 corresponding genes. After the comparison with the KEGG library, 1,340\u00a0genes, which participate in 19 types of metabolic pathways, were obtained. A total of 249 pathogenic factors and 2 disease islands were predicted. 2 CRISPR sites and 8 Cas sites were predicted. It can be seen from the evolutionary tree that <jats:italic>Salmonella Wandsworth MLST1498<\/jats:italic> and <jats:italic>Paratyphi B str<\/jats:italic>.<jats:italic>SPB7<\/jats:italic> are gathered together. We identified one resistance gene, namely, aac(6\u2019)\u2010Iaa accounting for aminoglycoside resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>Salmonella Wandsworth<\/jats:italic> isolated in this study is <jats:italic>Salmonella<\/jats:italic> group Q. Consequently, it is necessary to strengthen the understanding of clinical infections of <jats:italic>Salmonella Wandsworth<\/jats:italic> and carry out continuous monitoring and research.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23901","type":"journal-article","created":{"date-parts":[[2021,7,10]],"date-time":"2021-07-10T17:29:37Z","timestamp":1625938177000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Multilocus sequence typing analysis and second\u2010generation sequencing analysis of <i>Salmonella Wandsworth<\/i>"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8607-9669","authenticated-orcid":false,"given":"Chunling","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Taizhou Central Hospital (Taizhou University Hospital) Taizhou 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