{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T21:29:51Z","timestamp":1789594191279,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T00:00:00Z","timestamp":1628812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81372332"],"award-info":[{"award-number":["81372332"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572822"],"award-info":[{"award-number":["81572822"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972908"],"award-info":[{"award-number":["81972908"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,9]]},"abstract":"Abstract<\/jats:title>\n \n Objectives<\/jats:title>\n \n Thrombospondin 1 (\n THBS1)<\/jats:italic>\n is known to play a key role in tumor metastasis, and aberrant DNA methylation is one of the mechanisms regulating\n THBS1<\/jats:italic>\n . The present study investigated whether methylated\n THBS1<\/jats:italic>\n in circulating cell\u2010free DNA from preoperative peritoneal lavage fluid (PPLF) and peripheral blood could be used as a potential biomarker for predicting peritoneal dissemination in gastric cancer (GC) patients.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The status of\n THBS1<\/jats:italic>\n methylation was detected by quantitative methylation\u2010specific PCR (MSP) in tumor tissues, paired PPLF, and serum from 92 GC patients. The correlation between methylated\n THBS1<\/jats:italic>\n levels and peritoneal dissemination of GC was studied, and its diagnostic value for predicting peritoneal dissemination was clarified by the receiver operating characteristic (ROC) curve.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Aberrant\n THBS1<\/jats:italic>\n methylation in tumor tissues was significantly higher than that in paracancerous normal tissues (\n p<\/jats:italic>\n \u00a0<\u00a00.0001). No\n THBS1<\/jats:italic>\n methylation was found in 40 healthy controls, and partial methylation was detected in 3 of 48 patients with chronic non\u2010atrophic gastritis. The frequency of\n THBS1<\/jats:italic>\n \u00a0methylation in pairing PPLF and serum from 92 GC patients was 52.2% (48\/92) and 58.7% (54\/92), respectively. The results of methylated\n THBS1<\/jats:italic>\n in pairing PPLF and serum were similar to those of tumor tissues. Aberrant\n THBS1<\/jats:italic>\n methylation in tumor tissues and pairing PPLF or serum was closely related to peritoneal dissemination, tumor progression, and poor prognosis (all\n p<\/jats:italic>\n \u00a0<\u00a00.0001).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Circulating methylated\n THBS1<\/jats:italic>\n DNAs in PPLF\/serum may predict peritoneal dissemination, a potential poor prognostic factor for GC patients.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jcla.23936","type":"journal-article","created":{"date-parts":[[2021,8,14]],"date-time":"2021-08-14T02:59:47Z","timestamp":1628909987000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Circulating methylated\n THBS1<\/i>\n DNAs as a novel marker for predicting peritoneal dissemination in gastric cancer"],"prefix":"10.1002","volume":"35","author":[{"given":"Xuan\u2010Yu","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Pathophysiology School of Basic Medical Sciences Zhengzhou University Zhengzhou China"},{"name":"Experimental Research Centre Institute of Cancer and Basic Medicine (ICBM) Chinese Academy of Sciences Cancer Hospital of the University of Chinese Academy of Sciences Gongshu District, Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe\u2010Nan","family":"Ling","sequence":"additional","affiliation":[{"name":"Experimental Research Centre Institute of Cancer and Basic Medicine (ICBM) Chinese Academy of Sciences Cancer Hospital of the University of Chinese Academy of Sciences Gongshu District, Hangzhou China"},{"name":"Department of Hepatobiliary & Pancreatic Surgery The First Affiliated Hospital Zhejiang University School of Medicine Shangcheng District, Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian\u2010Lian","family":"Hong","sequence":"additional","affiliation":[{"name":"Experimental Research Centre Institute of Cancer and Basic Medicine (ICBM) Chinese Academy of Sciences Cancer Hospital of the University of Chinese Academy of Sciences Gongshu District, Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi\u2010Ming","family":"Yu","sequence":"additional","affiliation":[{"name":"Experimental Research Centre Institute of Cancer and Basic Medicine (ICBM) Chinese Academy of Sciences Cancer Hospital of the University of Chinese Academy of Sciences Gongshu District, Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology School of Basic Medical Sciences Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2155-1111","authenticated-orcid":false,"given":"Zhi\u2010Qiang","family":"Ling","sequence":"additional","affiliation":[{"name":"Experimental Research Centre Institute of Cancer and Basic Medicine (ICBM) Chinese Academy of Sciences Cancer Hospital of the University of Chinese Academy of Sciences Gongshu District, Hangzhou 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