{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T13:00:10Z","timestamp":1777381210604,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T00:00:00Z","timestamp":1628035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009094","name":"Russian Humanitarian Foundation","doi-asserted-by":"publisher","award":["21\u201015\u201000029"],"award-info":[{"award-number":["21\u201015\u201000029"]}],"id":[{"id":"10.13039\/100009094","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To test a novel method of assessment of platelet adhesion to a fibrinogen\u2010coated surface in whole blood under flow conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We developed a fluidic device that mimics blood flow in vessels. The method of detection of platelet adhesion is based on recording of a scattered laser light signal from a fibrinogen\u2010covered surface. Testing was performed in platelet\u2010rich plasma (PRP) and whole blood of healthy volunteers. Control measurements were performed, followed by tests with inhibition of platelet GPIIa\/IIIb and GPIb receptors. Then, the same testing sequence was performed in whole blood of persons with autoimmune thrombocytopenia and type 3 von Willebrand disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The change in intensity of scattered light was 2.7 (2.4; 4.1) times higher in whole blood (0.2\u00a0\u00b1\u00a00.08V, <jats:italic>n<\/jats:italic>\u00a0=\u00a07) than in PRP (0.05\u00a0\u00b1\u00a00.02\u00a0V, <jats:italic>n<\/jats:italic>\u00a0=\u00a07), <jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.01. The blocking of GP IIb\/IIIa receptors decreased the intensity of scattered light to 8.5 (6.5;12)%; the blocking of GPIb receptors decreased it to 34 (23;58)%, <jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.01. In the whole blood of a person with autoimmune thrombocytopenia, the inhibition of GPIb receptors decreased platelet adhesion, but no effect was observed in type 3 von Willebrand disease. Inhibition of platelet GPIIb\/IIIa receptors alone or combined inhibition of GPIb and GPIIb\/IIIa receptors resulted in almost total suppression of adhesion in both cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our system effectively registers platelet adhesion to a fibrinogen\u2010coated surface under controlled\u2010flow conditions and may successfully be applied to the investigation of platelet adhesion kinetics.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23939","type":"journal-article","created":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T19:36:32Z","timestamp":1628105792000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Kinetics of platelet adhesion to a fibrinogen\u2010coated surface in whole blood under flow conditions"],"prefix":"10.1002","volume":"35","author":[{"given":"Zufar A.","family":"Gabbasov","sequence":"first","affiliation":[{"name":"National Medical Research Centre for Cardiology of the Ministry of Health of the Russian Federation  Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuliya N.","family":"Avtaeva","sequence":"additional","affiliation":[{"name":"National Medical Research Centre for Cardiology of the Ministry of Health of the Russian Federation  Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan S.","family":"Melnikov","sequence":"additional","affiliation":[{"name":"National Medical Research Centre for Cardiology of the Ministry of Health of the Russian Federation  Moscow Russia"},{"name":"Institute of Biomedical Problems of Russian Academy of Sciences  Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey D.","family":"Okhota","sequence":"additional","affiliation":[{"name":"National Medical Research Centre for Cardiology of the Ministry of Health of the Russian Federation  Moscow 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