{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T19:09:05Z","timestamp":1788808145459,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,8,8]],"date-time":"2021-08-08T00:00:00Z","timestamp":1628380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The 5,10\u2010methylenetetrahydrofolate reductase (MTHFR) is an important enzyme of folate and methionine metabolism, which is expressed in human oocytes and preimplantation. Due to the involvement of MTHFR <jats:italic>in<\/jats:italic> female reproduction, <jats:italic>we<\/jats:italic> tend to evaluate the influence of MTHFR A1298C polymorphism on ovarian marker reserves such as serum anti\u2010M\u00fcllerian hormone (AMH) levels in women after in vitro fertilization (IVF)\/intracytoplasmic sperm injection (ICSI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 100 women, who underwent ART treatment due to male factor infertility, were recruited into this study. MTHFR A1298C polymorphism was detected by polymerase chain reaction\u2010restriction fragment length polymorphism (PCR\u2010RFLP) technique, and serum AMH concentrations were measured by an ultrasensitive enzyme\u2010linked immunosorbent assay (ELISA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Women with the CC genotype had higher AMH levels (4.15\u00a0\u00b1\u00a01.67\u00a0ng\/ml), albeit not significant, than carriers with other genotypes after ovarian stimulation. No significant differences existed in terms of miscarriage and live birth rates among different genotype groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The presence of the C mutant allele of the 1298 polymorphism in the MTHFR gene led to an increasing trend in serum AMH concentrations; however, the numbers of oocytes retrieved decreased in women with mutated genotypes. The influence of the MTHFR C677T polymorphism on embryo quality and pregnancy rate after ART cycles remains unclear.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23948","type":"journal-article","created":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T04:45:01Z","timestamp":1628484301000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The effect of A1298c polymorphism of the MTHFR gene on anti\u2010M\u00fcllerian hormone levels: experimental and\u00a0Web\u2010based\u00a0analysis"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-5918","authenticated-orcid":false,"given":"Seyedeh Zahra","family":"Shahrokhi","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine School of Allied Medical Sciences Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1022-0053","authenticated-orcid":false,"given":"Faranak","family":"Kazerouni","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine School of Allied Medical Sciences Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9234-5318","authenticated-orcid":false,"given":"Firouzeh","family":"Ghaffari","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Female Infertility Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine ACECR Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8866-0737","authenticated-orcid":false,"given":"Morteza","family":"Hadizadeh","sequence":"additional","affiliation":[{"name":"Physiology Research Center Institute of Basic and Clinical Physiology Sciences Kerman University of Medical Sciences Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8134-9268","authenticated-orcid":false,"given":"Zahra","family":"Zolfaghary","sequence":"additional","affiliation":[{"name":"Reproductive Epidemiology Research Center Royan Institute for Reproductive Biomedicine ACECR Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,8]]},"reference":[{"issue":"6","key":"e_1_2_8_2_1","first-page":"813","article-title":"Genetic predictors of controlled ovarian hyperstimulation: where do we stand today?","volume":"17","author":"Altm\u00e4e S","year":"2011","journal-title":"Hum Reprod"},{"issue":"4","key":"e_1_2_8_3_1","first-page":"268","article-title":"\u201canti\u2010mullerian hormone: Marker for ovarian response in controlled ovarian stimulation for IVF patients\u201d: A first pilot study in the Indian 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