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However, there is no relatively mature DNA isolation method or commercial kit available that is compatible with different LB sample types. This study developed a PAN\u2010sample DNA isolation method (PAN method) for liquid biopsy samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The PAN method has two key steps, including biosample\u2010specific pretreatments for various LB sample types and high concentration guanidine thiocyanate buffer for lysis and denaturation procedure. Subsequently, the performance of PAN method was validated by a series of molecular analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The PAN method was used to isolate DNA from multiple sample types related to LB, including plasma, serum, saliva, nasopharyngeal swab, and stool. All purified DNA products showed good quality and high quantity. Comparison of KRAS mutation analysis using DNA purified using PAN method versus QIAamp methods showed similar efficiency. Epstein\u2010Barr virus DNA was detected via Q\u2010PCR using DNA purified from serum, plasma, nasopharyngeal swab, and saliva samples collected from nasopharyngeal carcinoma patients. Similarly, methylation sequencing of swab and saliva samples revealed good coverage of target region and high methylation of HLA\u2010DPB1\u00a0gene. Finally, 16S rDNA gene sequencing of saliva, swab, and stool samples successfully defines the relative abundance of microbial communities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study developed and validated a PAN\u2010sample DNA isolation method that can be used for different LB samples, which can be applied to molecular epidemiological research and other areas.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.23962","type":"journal-article","created":{"date-parts":[[2021,8,16]],"date-time":"2021-08-16T18:36:45Z","timestamp":1629139005000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and validation of a flexible DNA extraction (PAN) method for liquid biopsy of multiple sample types"],"prefix":"10.1002","volume":"35","author":[{"given":"Xiao\u2010Xia","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Cancer Prevention Research Sun 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