{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T02:22:02Z","timestamp":1781835722861,"version":"3.54.5"},"reference-count":23,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T00:00:00Z","timestamp":1632355200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY20H200008"],"award-info":[{"award-number":["LY20H200008"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Recently, the rise of syphilitic seroresistance brings great confusion to the clinical diagnosis and treatment of syphilis, and no clear diagnostic marker has been found to distinguish syphilitic seroresistance from other progression of syphilis. This study evaluated the serum chemokines levels of CCL2, CXCL8, CXCL9, and CXCL10 and its correlation with blood routine, coagulation, and biochemical indexes in seroresistant syphilitic patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Serum levels of chemokines were quantitatively determined by Flow Cytometric Bead Array (CBA). The results expressed in pg\/ml. Clinical parameters were detected and analyzed according to the clinical laboratory standards. A correlation analysis was subsequently performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The seroresistant syphilitic patients increased significantly serum chemokines levels of CXCL8 (***<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001), CXCL9 (***<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001), and CXCL10 (**<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01) when compared to noninfected individuals, but the CCL2 was not statistically significant, and serum CXCL8\u00a0shows a strong association with platelets (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.51, **<jats:italic>p<\/jats:italic>\u00a0=\u00a00.004) and serum CXCL10 was significantly positively related to INR levels (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.49, **<jats:italic>p<\/jats:italic>\u00a0=\u00a00.007).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Increasing serum abnormalities in CXCL8, CXCL9, and CXCL10\u00a0level combining with platelets of peripheral blood and plasmatic INR in syphilis patients may be helpful for the diagnosis of serofast state.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24016","type":"journal-article","created":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T20:15:18Z","timestamp":1632428118000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["CXCL8, CXCL9, and CXCL10 serum levels increase in syphilitic patients with seroresistance"],"prefix":"10.1002","volume":"35","author":[{"given":"Xiaoyan","family":"Dong","sequence":"first","affiliation":[{"name":"Center of Clinical Laboratory Medicine The Zhejiang Provincial People\u2019s Hospital People\u2019s Hospital of Hangzhou Medical College Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8536-2080","authenticated-orcid":false,"given":"Junwu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Medicine The Zhejiang Provincial People\u2019s Hospital People\u2019s Hospital of Hangzhou Medical College Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9803-1096","authenticated-orcid":false,"given":"Fangfang","family":"Yang","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Medicine The Zhejiang Provincial People\u2019s Hospital People\u2019s Hospital of Hangzhou Medical College Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0502-4813","authenticated-orcid":false,"given":"Jinlin","family":"Liu","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Medicine The Zhejiang Provincial People\u2019s Hospital People\u2019s Hospital of Hangzhou Medical College Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumeng","family":"Peng","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Medicine The Zhejiang Provincial People\u2019s Hospital People\u2019s Hospital of Hangzhou Medical College Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3321-2176","authenticated-orcid":false,"given":"Yumei","family":"Ge","sequence":"additional","affiliation":[{"name":"Center of Clinical Laboratory Medicine The Zhejiang Provincial People\u2019s Hospital People\u2019s Hospital of Hangzhou Medical College Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,23]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40471-018-0138-z"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01153-18"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2018-053753"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2012-050622"},{"key":"e_1_2_11_6_1","first-page":"1","article-title":"Prevention CfDCa. Sexually transmitted diseases treatment guidelines, 2015","volume":"64","author":"Workowski K","year":"2015","journal-title":"MMWR Recomm Rep"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000001975"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/CM9.0000000000001175"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2014.13259"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2016.3178"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.05.008"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/1756-185X.12589"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-021-01582-4"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-020-00663-w"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21082927"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr483"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20092283"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33569"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2008.0027"},{"issue":"2","key":"e_1_2_11_20_1","first-page":"216","article-title":"Monocyte chemoattractant protein 1 and fractalkine play opposite roles in angiogenesis recruitment of different macrophage subtypes","volume":"11","author":"Chen L","year":"2018","journal-title":"Int J Ophthalmol"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2020.112289"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00220"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/vim.2020.0217"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.2741\/2853"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24016","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.24016","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T05:29:13Z","timestamp":1693200553000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.24016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,23]]},"references-count":23,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["10.1002\/jcla.24016"],"URL":"https:\/\/doi.org\/10.1002\/jcla.24016","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,23]]},"assertion":[{"value":"2021-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e24016"}}