{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:55:56Z","timestamp":1753869356592,"version":"3.41.2"},"reference-count":21,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:00:00Z","timestamp":1632960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Accurately measuring plasma aldosterone concentration is difficult but meaningful for primary aldosteronism (PA) diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, we developed an ultra\u2010performance liquid chromatography\u2010tandem mass spectrometry (UPLC\u2010MS\/MS) method for plasma aldosterone detection, evaluated its performance according to guidelines issued by CLSI, including detection limit, linearity, precision, and compared it with chemiluminescence immunoassay. Then, a reference range of plasma aldosterone in young people was established by using this method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The lower limit of quantitation (LOQ) was 10\u00a0pg\/ml. The mean recovery rates of analyte added to serum were 100.07\u2013102.05% in different concentrations. The linearity range was 20\u20132000\u00a0pg\/ml. Inter\u2010assay CVs were 2.20\u20133.97% at aldosterone concentrations of 65.66\u2013854.75\u00a0pg\/ml. The regression equation of UPLC\u2010MS\/MS (x) and chemiluminescence immunoassay (<jats:italic>y<\/jats:italic>) was <jats:italic>y<\/jats:italic>\u00a0=\u00a01.002x\u00a0+\u00a065.854 (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.9456, <jats:italic>n<\/jats:italic>\u00a0=\u00a0237). The reference range of plasma aldosterone detected by UPLC\u2010MS\/MS was 11.30\u2013363.82\u00a0pg\/ml in young people in South China, and there was no statistically significant difference in plasma aldosterone concentration between two genders.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In conclusion, UPLC\u2010MS\/MS can rapidly and accurately detect plasma aldosterone and is appropriate for clinical application.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24029","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T16:39:39Z","timestamp":1633019979000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Developing an ultra\u2010performance liquid chromatography\u2010tandem mass spectrometry for detecting aldosterone in human plasma"],"prefix":"10.1002","volume":"35","author":[{"given":"Wenbin","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenrong","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuzhe","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The Third Affiliated Hospital of Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihao","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The Third Affiliated Hospital of Sun Yat\u2010sen University Guangzhou 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