{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T20:07:59Z","timestamp":1788898079200,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T00:00:00Z","timestamp":1633305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY18H060009"],"award-info":[{"award-number":["LY18H060009"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Gout is an inflammatory arthritis and is characterized by the accumulation of deposited monosodium urate (MSU) crystals in the joints. miRNAs may act as key regulators of gout pathogenesis. The aim of our study was to explore the underlying role and molecular mechanism of miR\u20103146 in the formation of neutrophil extracellular traps (NETs) during the pathogenesis of gout.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The expression of miR\u20103146 and sirtuin 1 (SIRT1) was determined by real\u2010time reverse transcription\u2010polymerase chain reaction and Western blot, respectively. The luciferase reporter assay was performed to identify the targeting relationship between miR\u20103146 and SIRT1. Reactive oxygen species (ROS) production was detected by fluorescent staining. NETs formation was demonstrated via immunofluorescence staining and ELISA method. Gout model was induced in rats to verify the effects of miR\u20103146 inhibition on histopathological changes and NETs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Here, we found miR\u20103146 expression was dramatically increased in neutrophils of patients with gout, which was accompanied with the higher levels of NETs. MSU crystals significantly increased miR\u20103146 expression and ROS production in neutrophils. The NETs process was also triggered by MSU crystals. Furthermore, we verified the interaction between miR\u20103146 and SIRT1. Additionally, antagomir\u20103146\u2010based therapy effectively inhibited the formation of NETs in rats with gout.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings indicated that miR\u20103146\u2010mediated NETs formation may play a potential role in the pathogenesis of gout. These results suggested that miR\u20103146 could be used as a potential therapeutic target for the treatment of gout.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.24032","type":"journal-article","created":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T14:10:50Z","timestamp":1633529450000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["miR\u20103146 induces neutrophil extracellular traps to aggravate gout flare"],"prefix":"10.1002","volume":"35","author":[{"given":"Lizhen","family":"Shan","sequence":"first","affiliation":[{"name":"Department of Endocrinology and Metabolism The Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1124-2219","authenticated-orcid":false,"given":"Di","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic 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