{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:38:21Z","timestamp":1788781101370,"version":"build-2803163510"},"reference-count":14,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,10,18]],"date-time":"2021-10-18T00:00:00Z","timestamp":1634515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100016310","name":"Zhao Yi-Cheng Medical Science Foundation","doi-asserted-by":"publisher","award":["ZYYFY2019029"],"award-info":[{"award-number":["ZYYFY2019029"]}],"id":[{"id":"10.13039\/501100016310","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin City","doi-asserted-by":"publisher","award":["18ZXDBSY00140"],"award-info":[{"award-number":["18ZXDBSY00140"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin City","doi-asserted-by":"publisher","award":["18JCYBJC91700"],"award-info":[{"award-number":["18JCYBJC91700"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin City","doi-asserted-by":"publisher","award":["17JCQNJC11500"],"award-info":[{"award-number":["17JCQNJC11500"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770118"],"award-info":[{"award-number":["81770118"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870101"],"award-info":[{"award-number":["81870101"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770110"],"award-info":[{"award-number":["81770110"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81800120"],"award-info":[{"award-number":["81800120"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["8180011"],"award-info":[{"award-number":["8180011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>A deeper understanding of the pathogenesis of severe aplastic anemia (SAA) is urgently warranted to achieve better therapeutic\u00a0effects. The objective of this study was to investigate the phagocytosis of myeloid dendritic cell (mDC) in SAA patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Myeloid dendritic cells were induced in vitro from bone marrow mononuclear cells from 26 SAA patients and 12 normal controls (HCs). The phagocytosis of mDCs was detected by flow cytometry using FITC\u2010Dextran (40KD), and its correlation with the immune status and severity of the disease was analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The phagocytosis of mDC from untreated SAA patients was significantly stronger than that from complete remission group and HC group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). There was no statistical difference between the latter two groups (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05). The phagocytosis of mDC from SAA patients correlated positively with the concentration of interleukin (IL)\u20102 (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.389,<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), and IL\u20104 (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.556,<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), negatively with CD4<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup>ratio (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.421,<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). It also had negative correlations with the level of hemoglobin (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.393,<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), white blood cell (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.436,<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), platelet (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.431,<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), and reticulocyte (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.447,<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). The phagocytosis of mDC does not correlate with the response to IST.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The increased phagocytosis of mDC in untreated SAA patients may contribute to abnormal activation of T helper (Th) and subsequent cytotoxic T lymphocyte (CTL) activation in these patients. It may be involved in the immune pathogenesis of SAA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24063","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T23:14:59Z","timestamp":1634771699000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Myeloid dendritic cells in severe aplastic anemia patients exhibit stronger phagocytosis"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0788-7076","authenticated-orcid":false,"given":"Yingying","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengcheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Cardiology Tianjin Medical University General Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaquan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningyuan","family":"Ran","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zonghong","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612825666190313113601"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-010-0761-z"},{"issue":"12","key":"e_1_2_7_4_1","first-page":"1040","article-title":"In vitro induction of allo\u2010T lymphocytes proliferation by myeloid dendritic cells in patients with severe aplastic anemia","volume":"48","author":"Jun W","year":"2009","journal-title":"Chinese J Intern Med"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2009.07842.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2019000853"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/bcj.2011.6"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2017.176701"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-08-368308"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-11-469353"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-09-740845"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12116"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPH.0000000000001737"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"8789275","DOI":"10.1155\/2020\/8789275","article-title":"Bone marrow plasma cytokine signature profiles in severe aplastic anemia","volume":"2020","author":"Liu B","year":"2020","journal-title":"Biomed Res Int"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1413485"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24063","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.24063","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24063","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T09:26:08Z","timestamp":1725960368000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.24063"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,18]]},"references-count":14,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/jcla.24063"],"URL":"https:\/\/doi.org\/10.1002\/jcla.24063","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,18]]},"assertion":[{"value":"2021-06-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e24063"}}