{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:24:56Z","timestamp":1790270696316,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,10,28]],"date-time":"2021-10-28T00:00:00Z","timestamp":1635379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008091","name":"Golestan University of Medical Sciences","doi-asserted-by":"publisher","award":["960427092"],"award-info":[{"award-number":["960427092"]}],"id":[{"id":"10.13039\/501100008091","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Stroke<\/jats:italic>, either due to <jats:italic>ischemia<\/jats:italic> or <jats:italic>hemorrhage<\/jats:italic>, causes acute neurological damages to the brain. There is shortage of reliable biomarkers for <jats:italic>ischemic stroke<\/jats:italic> (IS), and we therefore investigated the serum concentrations of microRNA\u2010210 (miR\u2010210) and hypoxia inducible factor\u20101\u03b1 (HIF\u20101\u03b1), as possible diagnostic and\/or prognostic markers for IS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum samples were acquired from 52 IS patients and their healthy counterparts at five time points: upon admission, 24 and 48\u00a0h after admission, upon discharge and 3\u00a0months later. Serum levels of miR\u2010210 and HIF\u20101\u03b1 were respectively analyzed using real time RT\u2010PCR and ELISA. Diagnostic and prognostic accuracy tests were performed to assess the value of suggested biomarkers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IS patients demonstrated higher levels of serum HIF\u20101\u03b1 and lower miR\u2010210 in comparison to the healthy subjects. MiR\u2010210 was suggested to be a weak diagnostic biomarker at the time of admission (AUC\u00a0=\u00a00.61; <jats:italic>p<\/jats:italic>\u00a0=\u00a00.05), while HIF\u20101\u03b1 was an acceptable diagnostic marker for IS (AUC\u00a0=\u00a00.73; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.0001). The higher expression of miR\u2010210 and lower levels of HIF\u20101\u03b1 were associated with better survivals in IS patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Serum miR\u2010210 is a weak diagnostic marker of IS. Serum HIF\u20101\u03b1 is a better biomarker in diagnosing IS patients but further work in larger groups, including those with hemorrhagic stroke is necessary to confirm its diagnostic utility. Similarly, the prognostic potentiality of miR\u2010210 and HIF\u20101\u03b1 was acceptable but needs bigger sample size and longer follow\u2010up to be statistically confirmed.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24073","type":"journal-article","created":{"date-parts":[[2021,10,28]],"date-time":"2021-10-28T11:10:27Z","timestamp":1635419427000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["The lower expression of circulating miR\u2010210 and elevated serum levels of HIF\u20101\u03b1 in <i>ischemic stroke<\/i>; Possible markers for diagnosis and disease prediction"],"prefix":"10.1002","volume":"35","author":[{"given":"Mina","family":"Rahmati","sequence":"first","affiliation":[{"name":"Department of Biochemistry Metabolic Disorders Research Center Faculty of Medicine Golestan University of Medical Sciences Gorgan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon 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