{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T19:53:07Z","timestamp":1790452387206,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100016022","name":"Data Center of Management Science, National Natural Science Foundation of China - Peking University","doi-asserted-by":"publisher","award":["81660341"],"award-info":[{"award-number":["81660341"]}],"id":[{"id":"10.13039\/501100016022","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016022","name":"Data Center of Management Science, National Natural Science Foundation of China - Peking University","doi-asserted-by":"publisher","award":["81760369"],"award-info":[{"award-number":["81760369"]}],"id":[{"id":"10.13039\/501100016022","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016022","name":"Data Center of Management Science, National Natural Science Foundation of China - Peking University","doi-asserted-by":"publisher","award":["81860361"],"award-info":[{"award-number":["81860361"]}],"id":[{"id":"10.13039\/501100016022","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2019M653802"],"award-info":[{"award-number":["2019M653802"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Alveolar echinococcosis (AE) is a zoonotic disease caused by the larval stage of <jats:italic>Echinococcus\u00a0multilocularis<\/jats:italic> parasitizing in the human liver, causing local pathological changes in the liver and manifesting as hyperplasia, liver fibrosis, atrophy, degeneration, and necrosis. Here, we report a method that can simultaneously isolate hepatocytes and hepatic stellate cells (HSCs) from mice infected with <jats:italic>Echinococcus\u00a0multilocularis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A mouse model of AE was established. Hepatocytes and HSCs were isolated from mouse liver using a two\u2010step method combining in situ collagenase perfusion and gradient centrifugation. Expressions of Alb, Desmin, and \u03b1\u2010SMA were detected with immunofluorescence to identify the isolated hepatocytes and HSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The viability and purity of hepatocytes and HSCs both reached 90% or above. For hepatocytes, clear cell boundaries were observed, and the nuclei were round or oval, with clear nucleoli. There was a homogeneous distribution of the hepatocyte marker Alb in the cytoplasm of hepatocytes. Lipid droplets and Desmin expression were observed in the cytoplasm of freshly isolated HSCs. During the activation of HSCs, the lipid droplets gradually decreased and disappeared with a high expression of \u03b1\u2010SMA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hepatocytes and HSCs are simultaneously isolated. This may provide a research tool to investigate the interaction between hepatocytes and HSCs and to investigate the mechanism of <jats:italic>Echinococcus\u00a0multilocularis<\/jats:italic> infection\u2010induced liver pathological changes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24084","type":"journal-article","created":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T14:32:09Z","timestamp":1635777129000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["An improved experimental method for simultaneously isolating hepatocytes and hepatic stellate cells in mouse liver infected with <i>Echinococcus multilocularis<\/i>"],"prefix":"10.1002","volume":"35","author":[{"given":"Xiaojuan","family":"Bi","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogenesis Prevention, and Treatment of Central Asian High Incidence Diseases Clinical Medical Research Institute The First Affiliated Hospital of 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