{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T19:25:46Z","timestamp":1781983546938,"version":"3.54.5"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,11,6]],"date-time":"2021-11-06T00:00:00Z","timestamp":1636156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81873730"],"award-info":[{"award-number":["81873730"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Ferroptosis is a novel iron\u2010dependent form of cell death, which\u00a0is implicated in various diseases including cancers. However, the influence of ferroptosis\u2010related genes on the prognosis of breast cancer remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>RNA sequencing data of 1053 breast cancer tissue samples and 111 normal tissue samples from The Cancer Genome Atlas (TCGA) were analyzed. Expression levels of 259 ferroptosis\u2010related genes were compared. Gene Ontology (GO) and the Kyoto Gene and Genomic Encyclopedia (KEGG) analyses were conducted on differentially expressed genes. Cox univariate analysis was conducted to explore the potential prognostic biomarkers of breast cancer. Infiltrating immune cell status was assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 66 ferroptosis\u2010related genes were differentially expressed in breast cancer tissues. The enriched GO terms included Biological Process (mainly included response to oxidative stress, cellular response to chemical stress, multicellular organismal homeostasis, cofactor metabolic process, response to metal ion, response to steroid hormone, cellular response to oxidative stress, transition metal ion homeostasis, iron ion homeostasis, and cellular iron ion homeostasis), Cellular Component (mainly included apical plasma membrane, early endosome, apical part of cell, lipid droplet, basolateral plasma membrane, blood microparticle, clathrin\u2010coated pit, caveola, astrocyte projection, and pronucleus) and Molecular Function (mainly included iron ion binding, ubiquitin protein ligase binding, oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, oxidoreductase activity, acting on the CH\u2212OH group of donors, NAD or NADP as acceptor, ferric iron binding, aldo\u2212keto reductase (NADP) activity, oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, steroid dehydrogenase activity, alditol:NADP+1\u2212oxidoreductase activity, and alcohol dehydrogenase (NADP+) activity). The enriched KEGG pathway mainly included the HIF\u20101\u00a0signaling pathway, NOD\u2010like receptor signaling pathway, ferroptosis, IL\u201017\u00a0signaling pathway, central carbon metabolism in cancer, PPAR signaling pathway, PD\u2010L1 expression, and PD\u20101 checkpoint pathway in cancer. Among them, 38 ferroptosis\u2010related genes were significantly associated with the prognosis of breast cancer. The prognostic model was constructed, and breast cancer patients in low\u2010risk group had a better prognosis. In addition, risk score of ferroptosis prognostic model was negatively correlated with B cells (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.063, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.049), CD8+ T cells (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.083, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.010), CD4+ T cells (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.097, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.002), neutrophils (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.068, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.033), and dendritic cells (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.088, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.006).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The ferroptosis pathway plays a key role in breast cancer. Some differentially expressed ferroptosis\u2010related genes can be used as prognostic biomarkers for breast cancer.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24094","type":"journal-article","created":{"date-parts":[[2021,11,6]],"date-time":"2021-11-06T07:23:13Z","timestamp":1636183393000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The role of ferroptosis\u2010related genes for overall survival prediction in breast cancer"],"prefix":"10.1002","volume":"35","author":[{"given":"Li\u2010Yan","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Thyroid and breast surgery The Second Affiliated Hospital of Soochow University Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010lin","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Thyroid and breast surgery The Second Affiliated Hospital of Soochow University Suzhou 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