{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T07:29:42Z","timestamp":1770535782361,"version":"3.49.0"},"reference-count":14,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T00:00:00Z","timestamp":1636588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study is to determine the cut\u2010off value of glucose\u20106\u2010phosphate dehydrogenase (G6PD) activity and the mutation spectrum of <jats:italic>G6PD<\/jats:italic> gene in neonates with G6PD deficiency at Ningbo. Around 82233 neonatal blood samples were measured to determine G6PD activity. The positive samples were further detected with gene analysis. A total of 445 neonates were confirmed as G6PD deficiency, and the incidence in Ningbo was 1\/185. 17 types of <jats:italic>G6PD<\/jats:italic> gene mutations were found, including 11 single\u2010site mutations and 6 double\u2010site mutations. Considering the significant differences in G6PD activity, the cut\u2010off value was detected to be 2.35 and 3.65\u00a0U\/gHb for males and females, respectively. Significant differences in G6PD activities were noted and found to be varied from 4.61 to 6.02\u00a0U\/gHb in different seasons (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.0001). G6PD deficiency screening is a significant detection test for neonatal G6PD deficiency prevention. Our study highlights that the screening should be done using different cut\u2010off values according to the sexes in different seasons.<\/jats:p>","DOI":"10.1002\/jcla.24104","type":"journal-article","created":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T17:28:34Z","timestamp":1636651714000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular genotyping of G6PD mutations for neonates in Ningbo area"],"prefix":"10.1002","volume":"35","author":[{"given":"Jiewen","family":"Pan","sequence":"first","affiliation":[{"name":"Central Laboratory of Birth Defects Prevention and Control Ningbo Women &amp; Children\u2019s Hospital Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danyan","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Central Laboratory of Birth Defects Prevention and Control Ningbo Women &amp; Children\u2019s Hospital Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Yu","sequence":"additional","affiliation":[{"name":"Central Laboratory of Birth Defects Prevention and Control Ningbo Women &amp; Children\u2019s Hospital Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Pan","sequence":"additional","affiliation":[{"name":"Central Laboratory of Birth Defects Prevention and Control Ningbo Women &amp; Children\u2019s Hospital Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youwei","family":"Bao","sequence":"additional","affiliation":[{"name":"Central Laboratory of Birth Defects Prevention and Control Ningbo Women &amp; Children\u2019s Hospital Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuqing","family":"Pan","sequence":"additional","affiliation":[{"name":"Central Laboratory of Birth Defects Prevention and Control Ningbo Women &amp; Children\u2019s Hospital Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Wang","sequence":"additional","affiliation":[{"name":"Central Laboratory of Birth Defects Prevention and Control Ningbo Women &amp; Children\u2019s Hospital Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisha","family":"Ge","sequence":"additional","affiliation":[{"name":"Central Laboratory of Birth Defects Prevention and Control Ningbo Women &amp; Children\u2019s Hospital Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9082-0488","authenticated-orcid":false,"given":"Haibo","family":"Li","sequence":"additional","affiliation":[{"name":"Central Laboratory of Birth Defects Prevention and Control Ningbo Women &amp; Children\u2019s Hospital Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,11]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"1045","article-title":"Neonatal screening program for G6PD deficiency in India: Need And Feasibility","volume":"46","author":"Nair H","year":"2009","journal-title":"Indian Pediatr"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2016.1220304"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2008.12.005"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019-557X.157537"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.908402"},{"key":"e_1_2_8_7_1","first-page":"415","article-title":"Detection of glucose\u20106\u2010phosphate dehydrogenase gene mutations of Tujia ethnic in Jiangkou, Guizhou","volume":"26","author":"Wu CX","year":"2007","journal-title":"Chinese J Endemiol"},{"key":"e_1_2_8_8_1","first-page":"51","article-title":"Analysis of the glucose\u20106\u2010phosphate dehydrogenase mutations in Guang Dong province and the relationship with clinical presentations","volume":"13","author":"Zhang X","year":"2000","journal-title":"J Clin Hematol"},{"key":"e_1_2_8_9_1","first-page":"1329","article-title":"The glucose\u20106\u2010phosphate dehydrogenase gene mutation and the clinical manifestation in Guangxi","volume":"22","author":"Zheng M","year":"2007","journal-title":"Matern Child Health Care China"},{"key":"e_1_2_8_10_1","first-page":"143","article-title":"Researches in gene mutations of G6PD deficiency in China in recent years","volume":"14","author":"Xu Y","year":"2009","journal-title":"J China Pediat Blood Cancer"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1017"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2016.07.006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V81.8.2150.2150"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000011553"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2010.090104"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24104","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.24104","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T03:08:37Z","timestamp":1693192117000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.24104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,11]]},"references-count":14,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/jcla.24104"],"URL":"https:\/\/doi.org\/10.1002\/jcla.24104","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,11]]},"assertion":[{"value":"2020-08-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e24104"}}