{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T22:54:02Z","timestamp":1791672842156,"version":"4.3.4"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T00:00:00Z","timestamp":1639008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Myocardial infarction (MI) is a serious cardiovascular disease associated with myocardial ischemia\/reperfusion (I\/R) injury. Dexmedetomidine (Dex), an \u03b12\u2010adrenoceptor agonist, has been reported to protect against I\/R injury. We examined the cardioprotective effects of Dex on cardiomyocytes under hypoxia\/reoxygenation (H\/R) conditions and explored the underlying mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>A H\/R model was established to mimic the MI injury. The CCK\u20108 assay was performed to measure cell viability. Cellular apoptosis was measured using the Annexin V fluorescein isothiocyanate (FITC)\u2010propidium iodide (PI) staining. The levels of interleukin (IL)\u20101\u03b1 and tumor necrosis factor (TNF)\u2010\u03b1, and the activity of lactate dehydrogenase (LDH) were measured using a commercial enzyme\u2010linked immunosorbent assay (ELISA) kit. Reactive oxygen species (ROS) were measured using the 2'\u20107\u2019 dichlorofluorescein diacetate (DCFH\u2010DA) staining assay. In addition, the levels of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD), catalase (CAT), and caspase\u20103 were measured using a commercial kit. siRNA was used to silence Bcl\u20102, catalase, or STAT3. Western blotting was used to measure the change in the levels of proteins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Dex improved the cell viability and inhibited the inflammatory response in H9c2 cells exposed to H\/R treatment. In addition, Dex inhibited apoptosis and alleviated the endoplasmic reticulum (ER) stress and oxidative stress in H9c2 cells under the H\/R treatment. Mechanism investigation showed that Dex inhibited the intrinsic pathway of apoptosis. Moreover, Dex enhanced the activation of the JAK2\/STAT3\u00a0signaling pathway in H\/R\u2010treated H9c2 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Altogether, our findings suggested Dex as a promising therapeutic agent for myocardial I\/R.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.24119","type":"journal-article","created":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T09:07:16Z","timestamp":1639040836000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Dexmedetomidine protects cardiomyocytes against hypoxia\/reoxygenation injury via multiple mechanisms"],"prefix":"10.1002","volume":"36","author":[{"given":"Shunv","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Anesthesiology The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junbo","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)  Hangzhou Zhejiang 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