{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T15:30:36Z","timestamp":1757777436305,"version":"3.41.2"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,3,11]],"date-time":"2022-03-11T00:00:00Z","timestamp":1646956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Steroid\u2010induced osteonecrosis of femoral head (SONFH) is a serious complication of glucocorticoid overused. Recent evidence has demonstrated that circRNAs exert key pathophysiological roles in a variety of disease processes. However, the role of circRNA in SONFH remains largely unknown. The current study sought to evaluate how hsa_circ_0058122 affects SONFH in dexamethasone (DEX) treated human umbilical vein endothelial cells (HUVECs) model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>RT\u2010PCR was used to demonstrate the hsa_circ_0058122 expression level in Dex\u2010treated HUVECs cells. The effects of hsa_circ_0058122 on HUVECs apoptosis were evaluated via overexpression plasmid and siRNA. Using dual\u2010luciferase and fluorescence in situ hybridization assays, we demonstrated that hsa_circ_0058122 binds to miR\u20107974 thereby facilitating HUVECs apoptosis. Bioinformatics analysis and western blot were performed to confirm target genes of hsa\u2010miR\u20107974.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In our previous work, we revealed the top 20 elevated circRNAs in SONFH patients were hsa_circ_0010027, hsa_circ_0058115, hsa_circ_0010026, hsa_circ_0058839, hsa_circ_0056886, hsa_circ_0056885, hsa_circ_0058146, hsa_circ_0058105, hsa_circ_0058112, hsa_circ_0058143, hsa_circ_0058102, hsa_circ_0058090, hsa_circ_0075353, hsa_circ_0058126, hsa_circ_0058130, hsa_circ_0058140, hsa_circ_0058122, hsa_circ_0058123, hsa_circ_0058103, and hsa_circ_0058121. Among these, hsa_circ_0058122 was finally selected for further investigation. We found hsa_circ_0058122 expression was markedly elevated in Dex\u2010treated HUVECs cells, and the Dex\u2010mediated HUVEC apoptosis was impaired in hsa_circ_0058122\u2010silenced cells and increased in hsa_circ_0058122\u2010overexpressing cells. hsa_circ_0058122 competitively binds to hsa\u2010miR\u20107974, which in turn interacts with insulin\u2010like growth factor binding protein 5 (IGFBP5).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>hsa_circ_0058122\/miR\u20107974\/IGFBP5 was proposed to be a key regulatory pathway for SONFH. DEX treatment upregulated hsa_circ_0058122 expression in HUVECs, which sponged miR\u20107974, thereby increasing IGFBP5 expression, the hsa_circ_0058122\/miR\u20107974\/IGFBP5 axis contributed to the Dex\u2010mediated apoptosis. These findings may identify novel targets for SONFH molecular therapy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24134","type":"journal-article","created":{"date-parts":[[2022,3,11]],"date-time":"2022-03-11T11:13:50Z","timestamp":1646997230000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["hsa_circ_0058122 knockdown prevents steroid\u2010induced osteonecrosis of the femoral head by inhibiting human umbilical vein endothelial cells apoptosis via the miR\u20107974\/IGFBP5 axis"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2934-6668","authenticated-orcid":false,"given":"Tao","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Orthopaedics The Third Affiliated Hospital of Anhui Medical University The First People's Hospital of Hefei  Hefei 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