{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T23:51:33Z","timestamp":1789343493235,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T00:00:00Z","timestamp":1641600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cisplatin is a commonly used chemotherapeutic drug in clinics, and long\u2010term application will lead to hearing impairment. LLY\u2010283, an inhibitor of PRMT5, has not been reported in deafness. Our study aimed to explore the mechanism of LLY\u2010283 in hearing impairment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>First, we performed RNA\u2010seq (cisplatin in the experimental group and DMSO in the control group) to obtain the biological processes mainly involved in differentially expressed genes (DEGs). CCK\u20108 and LDH experiments were used to observe the effect of LLY\u2010283 on cisplatin\u2010induced auditory cell injury. ROS experiment was used to monitor the impact of LLY\u2010283 on oxidative damage of auditory cells. Effect of LLY\u2010283 on apoptosis of auditory cells detected by TUNEL experiment. PCR and Western blotting were used to detect the expression of genes and proteins related to auditory cell apoptosis in LLY\u2010283 cells. Meanwhile, we explored the effect of LLY\u2010283 on the expression of PRMT5 in cisplatin\u2010induced hearing impaired cells at RNA and protein levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Biological process analysis showed that DEGs were mainly enriched in the apoptotic process involved in morphogenesis (\u2010Log<jats:sub>10<\/jats:sub> <jats:italic>P<\/jats:italic>\u00a0=\u00a03.71). CCK\u20108 and LDH experiments confirmed that LLY\u2010283 could save cisplatin\u2010induced auditory cell injury. ROS experiments confirmed that LLY\u2010283 could rescue cisplatin\u2010induced oxidative damage to auditory cells. TUNEL experiments confirmed that LLY\u2010283 could protect cisplatin\u2010induced apoptosis of auditory cells. Meanwhile, LLY\u2010283 could inhibit the expression of PRMT5 in auditory cells induced by cisplatin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>LLY\u2010283 can rescue cisplatin\u2010induced auditory cell apoptosis injury. LLY\u2010283 can inhibit the increase in PRMT5 expression induced by cisplatin.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24176","type":"journal-article","created":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T10:31:49Z","timestamp":1641637909000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Explore the effect of LLY\u2010283 on the ototoxicity of auditory cells caused by cisplatin: A bioinformatic analysis based on RNA\u2010seq"],"prefix":"10.1002","volume":"36","author":[{"given":"Bin","family":"Zhao","sequence":"first","affiliation":[{"name":"Xiamen Health and Medical Big Data Center  Xiamen China"},{"name":"School of Medicine Xiamen University  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongdong","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medicine Xiamen University  Xiamen China"},{"name":"Department of Otolaryngology\u2010Head and 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