{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T02:27:52Z","timestamp":1783391272271,"version":"3.54.6"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T00:00:00Z","timestamp":1640649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Diabetic nephropathy (DN), a common microvascular complication of type 2 diabetes mellitus (T2DM), is an important factor causing chronic kidney disease. However, the relationship between miR\u201029a and DN remains unknown. Therefore, a cross\u2010sectional study was conducted to identify a potential molecular biomarker for DN prevention and management by detecting the serum miR\u201029a levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The serum miR\u201029a levels were measured in 360\u00a0subjects (180\u00a0T2DM patients and 180\u00a0healthy controls) using quantitative reverse transcription PCR (qRT\u2010PCR), and other conventional indicators were measured and analysed. A binary logistic regression was used to evaluate the DN risk factors; a receiver operating characteristic (ROC) curve was applied to analyse the diagnostic efficacy of miR\u201029a for DN, and a Spearman's rank correlation analysis was used to evaluate the correlation between serum miR\u201029a and cystatin C.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The serum miR\u201029\u00a0levels in the T2DM patients were higher than those in the healthy subjects and significantly increased with the progression of DN (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05). Serum miR\u201029a and cystatin C are independent predictors of the occurrence of DN. Compared with a single indicator, the combination of serum miR\u201029a and cystatin C has better DN diagnostic performance. In addition, the serum miR\u201029a levels were positively correlated with cystatin C in the patients with DN (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.521, <jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The expression of serum miR\u201029a was significantly associated with the occurrence and progression of DN and is expected to become a potential biomarker for the diagnosis of DN.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24210","type":"journal-article","created":{"date-parts":[[2021,12,29]],"date-time":"2021-12-29T07:41:00Z","timestamp":1640763660000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Significance of serum miR\u201029a in the occurrence and progression of diabetic nephropathy: A cross\u2010sectional 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